Using of group-based selected flavonoids as alternative inhibitors for potato polyphenol oxidase

One of the main problems encountered after fresh-cutting of foods is enzymatic browning causing changes in the texture, taste, and color. The variety of physical and chemical-based antibrowning methods was applied to extend the shelf life of these products. Accordingly, methods using natural compounds are of great importance for health. In this study, it was aimed to prevent enzymatic browning in potato (Solanum tuberosum L. cv. Marfona) by limiting the polyphenol oxidase (PPO) activity with selected flavonoids. First of all, we purified the potato PPO (pPPO) by single-step affinity chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and native PAGE were applied on pPPO. Then, the inhibition effects of 7-hydroxyflavone, 7-hydroxy-4 '-nitroisoflavone, myricetin, luteolin, 7-methoxyflavone, 6-fluoroflavone, diosmetin, rutin, and diosmin on purified PPO enzyme were investigated. Kinetic assays indicated that myricetin was a remarkable inhibitor with the K-i value of 5 mu M on pPPO.