Inhibitor Effects of Some Artificial Tears Drops on Human Carbonic Anhydrase Enzyme-II (hCA-II)
Özet
Carbonic anhydrases (CAs) are a metalloenzyme that contains zinc ions in their active sites, and they reversibly catalyze the reactions of hydration of CO2 and dehydration of HCO3-in the living. In this study, human carbonic anhydrase II (hCAII) isoenzyme was purified from human erythrocytes by affinity column (Sepharose 4B-L-tyrosine-sulfanilamide). The inhibitory effect of artificial tear drops on hCAII isoenzyme was investigated. IC50 values were calculated as 1.01 µL for sodium hyaluronate, 140.06 µL for polyvinyl alcohol+povidone, and 49.51 for polyethylene glycol+propylene glycol. The Ki value for sodium hyaluronate was found as 0.339±0.121 mM. According to these results, sodium hyaluronate was the most effective inhibitor in terms of application volume (microliter) among the three drugs examined.
Atıf için künye
ÇALIŞKAN, B., & Aksoy, M. (2023). Inhibitor Effects of Some Artificial Tears Drops on Human Carbonic Anhydrase Enzyme-II (hCA-II). Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 13(2), 941–947. https://doi.org/10.21597/jist.1253344
BibTeX
@article{caliskan2023-56801,
author = {ÇALIŞKAN, Büşra and Aksoy, Mine},
title = {Inhibitor Effects of Some Artificial Tears Drops on Human Carbonic Anhydrase Enzyme-II (hCA-II)},
journal = {Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi},
year = {2023},
volume = {13},
number = {2},
pages = {941--947},
issn = {2146-0574},
doi = {10.21597/jist.1253344},
url = {https://akademik.atauni.edu.tr/yayinlar/56801-inhibitor-effects-of-some-artificial-tears-drops-on-human-carbonic-anhydrase}
}