Conducting Polymer-Reduced Graphene Oxide Sponge Electrode for Electrochemical Detection Based on DNA Hybridization

Dağcı Kıranşan, Kader; Topçu, Ezgi

doi:10.1021/acsanm.0c00782

Makale Tam Makale Uluslararası 2020

Conducting Polymer-Reduced Graphene Oxide Sponge Electrode for Electrochemical Detection Based on DNA Hybridization

Kader Dağcı Kıranşan, Ezgi Topçu

ACS APPLIED NANO MATERIALS Cilt 3 · Sayı 6 · ss. 5449–5462 · 01.06.2020

DOI ile aç

Özet

In this study, polymer-supported graphene-based material was designed for electrochemical determination based on the hybridization of DNA. First, dispersions containing graphene oxide (GO) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) polymer in different compositions were transformed into a hydrogel form through the hydrothermal treatment, then 3D and flexible PEDOT:PSS/reduced graphene oxide (RGO) composite sponge material was prepared by using a freeze-drying process. Structural and morphological characterizations showed that PEDOT:PSS polymer served as a skeleton by covering the graphene layers like a mesh and provided an increment in both mechanical strength and electrochemical performance by relatively reducing pore size in 3D material. PEDOT:PSS/RGO composite sponge has high conductivity (158 S cm(-1)) for utilization as an electrode and durability to lift 700 times its weight. For electrochemical determination based on the hybridization of DNA on PEDOT:PSS/RGO composite sponge material, single-stranded DNA (ssDNA) and methylene blue (MB) marked ssDNA probes were immobilized on PEDOT:PSS/RGO composite sponge material at optimum conditions. This sensor achieved a detection limit down to 17 fM with the linear range of 50 fM to 2 mu M for the determination of hybridized DNA. By possessing flexible, free-standing, and durable features, PEDOT:PSS/RGO composite sponge appeared to be a promising electrode material for detection studies.

Anahtar kelimeler

FILMS BINDING adsorption FABRICATION Reduced graphene oxide 3-DIMENSIONAL GRAPHENE HIGH-PERFORMANCE METHYLENE-BLUE Sponge electrode conductive polymer DNA detection INITIATED ENZYMATIC POLYMERIZATION LABEL-FREE PAPER ELECTRODE COMPOSITE PAPER

Atıf için künye

Dağcı Kıranşan, K., & Topçu, E. (2020). Conducting Polymer-Reduced Graphene Oxide Sponge Electrode for Electrochemical Detection Based on DNA Hybridization. ACS APPLIED NANO MATERIALS, 3(6), 5449–5462. https://doi.org/10.1021/acsanm.0c00782

BibTeX

@article{dagcikiransan2020-55233,
  author = {Dağcı Kıranşan, Kader and Topçu, Ezgi},
  title = {Conducting Polymer-Reduced Graphene Oxide Sponge Electrode for Electrochemical Detection Based on DNA Hybridization},
  journal = {ACS APPLIED NANO MATERIALS},
  year = {2020},
  volume = {3},
  number = {6},
  pages = {5449--5462},
  issn = {2574-0970},
  doi = {10.1021/acsanm.0c00782},
  url = {https://akademik.atauni.edu.tr/yayinlar/55233-conducting-polymer-reduced-graphene-oxide-sponge-electrode-for-electrochemical}
}