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Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathway

Kübra Koç, Nihal Şimşek Özek, Ferhunde Aysin, Özlem Demir, Aslı Yılmaz, Mehmet Yılmaz, Fatime Geyikoğlu, Huseyin Serkan Erol

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH Cilt 34 · Sayı 2 · ss. 755–766 · 01.01.2024
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Özet

This study investigates the protective role of Hispidulin on acute respiratory distress syndrome (ARDS) in rats. Rats were divided into three groups: control, ARDS, ARDS+ Hispidulin. The ARDS models were established by injecting rats with oleic acid. Hispidulin (100 mg/kg) was injected i.p. an hour before ARDS. Myeloperoxidase (MPO), Interleukin-8 (IL-8), Mitogen-activated protein kinases (MAPK), Lipid Peroxidation (LPO), Superoxide Dismutase (SOD), Glutathione (GSH), and Angiotensin-converting enzyme (ACE) were determined by ELISA. Tumor necrosis factor-alpha (TNF-alpha) expression was described by RT-qPCR. Caspase-3 immunostaining was performed to evaluate apoptosis. Compared with the model group, a significant decrease was observed in the MPO, IL-8, MAPK, ACE, LPO levels, and TNF-alpha expression in the ARDS+ Hispidulin group. Moreover, reduced caspase-3 immunoreactivity and activity of ACE were detected in the Hispidulin+ARDS group. The protective effect of Hispidulin treatment may act through inhibition of the ACE activity and then regulation of inflammatory cytokine level and alteration of apoptosis.

Anahtar kelimeler

PATHOGENESIS oxidative stress mice apoptosis FATTY-ACIDS ACUTE LUNG INJURY respiratory distress syndrome Hispidulin lung injury with ARDS ACE activity

Atıf için künye

Koç, K., Şimşek Özek, N., Aysin, F., Demir, Ö., Yılmaz, A., Yılmaz, M., Geyikoğlu, F., & Erol, H. S. (2024). Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathway. INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH, 34(2), 755–766. https://doi.org/10.1080/09603123.2023.2166023

BibTeX 
@article{koc2024-53728,
  author = {Koç, Kübra and Şimşek Özek, Nihal and Aysin, Ferhunde and Demir, Özlem and Yılmaz, Aslı and Yılmaz, Mehmet and Geyikoğlu, Fatime and Erol, Huseyin Serkan},
  title = {Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathway},
  journal = {INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH},
  year = {2024},
  volume = {34},
  number = {2},
  pages = {755--766},
  issn = {0960-3123},
  doi = {10.1080/09603123.2023.2166023},
  url = {https://akademik.atauni.edu.tr/yayinlar/53728-hispidulin-exerts-a-protective-effect-against-oleic-acid-induced-ards-in-the-rat}
}