Purification and Properties of Peroxidase from Prangos ferulacea (Apiaceae) and Investigation of Inhibition by Some Chemicals
Özet
A peroxidase (POD)-containing fraction was purified from Prangos ferulacea (apiaceae) by (NH4)(2)SO4 precipitation and anion exchange chromatography. The purification grade obtained was 126.36 yielding 41.8% of the enzyme activity. The molecular weight was estimated to be 40 kDa by gel filtration. It has an optimum pH of 5 and exhibited high pH and thermal stabilities. Km for guaiacol and ABST were 1.347 and 1 mM, respectively. The optimal temperature was 30 degrees C. The 150 values for KSCN and NaN3 were 0.750 mM, 1.321 mM in Prangos ferulacea (Apiaceae) CA, respectively.
Atıf için künye
Nadaroğlu, H. (2009). Purification and Properties of Peroxidase from Prangos ferulacea (Apiaceae) and Investigation of Inhibition by Some Chemicals. ASIAN JOURNAL OF CHEMISTRY, 21(7), 5768–5776.
BibTeX
@article{nadaroglu2009-330261,
author = {Nadaroğlu, Hayrunnisa},
title = {Purification and Properties of Peroxidase from Prangos ferulacea (Apiaceae) and Investigation of Inhibition by Some Chemicals},
journal = {ASIAN JOURNAL OF CHEMISTRY},
year = {2009},
volume = {21},
number = {7},
pages = {5768--5776},
issn = {0970-7077},
url = {https://akademik.atauni.edu.tr/yayinlar/330261-purification-and-properties-of-peroxidase-from-prangos-ferulacea-apiaceae-and}
}