Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane

DEMİR, YAŞAR; DEMİR, NAZAN; Nadaroğlu, Hayrunnisa; BAKAN, Ebubekir

doi:10.1080/10826060008544944

Makale Tam Makale Uluslararası 2000

Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane

YAŞAR DEMİR, NAZAN DEMİR, Hayrunnisa Nadaroğlu, Ebubekir BAKAN

Preparative biochemistry & biotechnology Cilt 30 · Sayı 1 · ss. 49–59 · 01.01.2000

DOI ile aç

Özet

Carbonic anhydrase (CA) was purified from bovine erythrocyte plasma membrane and characterized in this study. For this purpose, the blood taken from young:animals was hemolysed, the membrane fraction was separated, and this fraction was repeatedly washed. The enzyme (CA) was removed from the membrane with buffered TritonX-100 (1%); it could be purified at a factor of 22.8 by affinity chromatography.

Atıf için künye

DEMİR, Y., DEMİR, N., Nadaroğlu, H., & BAKAN, E. (2000). Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane. Preparative biochemistry & biotechnology, 30(1), 49–59. https://doi.org/10.1080/10826060008544944

BibTeX

@article{demir2000-330217,
  author = {DEMİR, YAŞAR and DEMİR, NAZAN and Nadaroğlu, Hayrunnisa and BAKAN, Ebubekir},
  title = {Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane},
  journal = {Preparative biochemistry &amp; biotechnology},
  year = {2000},
  volume = {30},
  number = {1},
  pages = {49--59},
  issn = {1082-6068},
  doi = {10.1080/10826060008544944},
  url = {https://akademik.atauni.edu.tr/yayinlar/330217-purification-and-characterization-of-carbonic-anhydrase-from-bovine-erythrocyte}
}