A new Longidoridae member, Xiphinema brevicolle, from citrus orchards in Türkiye

Individuals of the genus Xiphinema were recovered from soil samples collected during citrus surveys in south-eastern Türkiye. Morphological examination using light microscopy identified the species as X. brevicolle. The female individuals measured 2000–2050 μm in length, and displayed a slightly flattened lip region, an amphidelphic reproductive system with two well-developed branches, and a short conoid tail ending in a rounded tip. These morphometric and morphological features were consistent with those of previously described X. brevicolle specimens. For molecular confirmation, the D2–D3 segment of the 28S rDNA was amplified using the D2AD3B primer pair, and sequenced; the resulting sequence has been deposited in GenBank (accession number PQ647284). Sequence analysis revealed a 95% identity with other X. brevicolle sequences in GenBank. Phylogenetic analysis, including neighbour-joining and maximum likelihood, was also performed. This study represents the first record of X. brevicolle from citrus orchards in Türkiye, extending the known geographical distribution of this species.