Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers

HALİLOĞLU, Kamil; Türkoğlu, Aras; Öztürk, Ali; Niedbała, Gniewko; Niazian, Mohsen; Wojciechowski, Tomasz; Piekutowska, Magdalena

doi:10.3390/agronomy13010255

Makale Tam Makale Uluslararası 2023 Açık erişimli

Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers

Kamil HALİLOĞLU, Aras Türkoğlu, Ali Öztürk, Gniewko Niedbała, Mohsen Niazian, Tomasz Wojciechowski, Magdalena Piekutowska

Agronomy Cilt 13 · Sayı 1 · 01.01.2023

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Özet

© 2023 by the authors.This study investigated the genetic diversity and population structure of 63 genotypes from Turkish bread wheat germplasm using iPBS-retrotransposons primers. The thirty-four iPBS primers produced a total of 1231 polymorphic bands, ranging from 8 (iPBS-2375) to 60 (iPBS-2381) alleles per marker, with an average number of 36.00 alleles. The polymorphism information content (PIC) per marker varied between 0.048 (iPBS 2087) and 0.303 (iPBS 2382), with an average of 0.175. The numbers of effective alleles (ne), genetic diversity of Nei (h), and Shannon’s information index (I) value were calculated as 1.157, 0.95, and 0.144, respectively. The greatest genetic distance (0.164) was between Eastern Anatolia Agricultural Research Institute genotypes and Çukurova Agricultural Research Institute genotypes. The unweighted pair-group method with arithmetic mean (UPGMA) dendrogram placed the 63 wheat genotypes into three clusters. The percentage of genetic diversity explained by each of the three main coordinates of the basic coordinate analysis was determined to be 44.58, 12.08, and 3.44, respectively. AMOVA (Analysis of Molecular Variance) showed that the variation within populations was 99% and that between populations was 1%. The result of genetic structure analysis suggests that the greatest value of K was calculated as 3. The F-statistic (Fst) value was determined as 0.4005, 0.2374, and 0.3773 in the first to third subpopulations, respectively. Likewise, the expected heterozygosity values (He) were determined as 0.2203, 0.2599, and 0.2155 in the first, second, and third subpopulations, respectively. According to the information obtained in the study, the most genetically distant genotypes were the G1 (Aksel 2000) and G63 (Karasu 90) genotypes. This study provided a deep insight into genetic variations in Turkish bread wheat germplasm using the iPBS-retrotransposons marker system.

Anahtar kelimeler

ASSOCIATION SITE Turkey L. GENETIC DIVERSITY wheat WINTER-WHEAT WILD DOMESTICATION GERMPLASM VARIETIES DROUGHT RESISTANCE GENOTYPES landraces PCoA Environmental conditions inter-primer binding site AMOVA

Atıf için künye

HALİLOĞLU, K., Türkoğlu, A., Öztürk, A., Niedbała, G., Niazian, M., Wojciechowski, T., & Piekutowska, M. (2023). Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers. Agronomy, 13(1). https://doi.org/10.3390/agronomy13010255

BibTeX

@article{haliloglu2023-284067,
  author = {HALİLOĞLU, Kamil and Türkoğlu, Aras and Öztürk, Ali and Niedbała, Gniewko and Niazian, Mohsen and Wojciechowski, Tomasz and Piekutowska, Magdalena},
  title = {Genetic Diversity and Population Structure in Bread Wheat Germplasm from Türkiye Using iPBS-Retrotransposons-Based Markers},
  journal = {Agronomy},
  year = {2023},
  volume = {13},
  number = {1},
  issn = {2073-4395},
  doi = {10.3390/agronomy13010255},
  url = {https://akademik.atauni.edu.tr/yayinlar/284067-genetic-diversity-and-population-structure-in-bread-wheat-germplasm-from-turkiye}
}