Molecular identification of Bacillus pumilus isolated from natural resources by 16S rRNA, chitinase enzyme activity and the industrial relevance of the related enzyme
Özet
The chitinase
which is identified in the literature is also known with is chemical name
β-1,4-N- acetyl glucosamine. Chitinases are produced by plants, animals and
microorganisms. Microbial-derived chitinases are preferred for biotechnological
applications. Chitinases are preferred in food industry, feed additives, paper
industry and soil improvement. In biotechnology, chitinase enzyme studies are
an effective tool to be used in the production of enzymes, or in the
development of their properties. <o:p></o:p>
The aim of this
study was to investigate the molecular identification of Bacillus pumilus strain isolated from fatty cheese by 16S rRNA, to
determine its chitinase enzyme activity and to evaluate its possible industrial
effectiveness. The identification was performed using the 16S rRNA method. The
identification rate of Bacillus pumilus was
99 % and the optimum temperature of the extracellular chitinase enzyme was
determined as 40°C and the optimum pH was pH 7.0. According to the results, it
has been confirmed with our study that the bacterium can be introduced to the
industrial use due to the characteristics of the enzyme produced by this
bacterium and that it can be used as a starter culture in the food industry. <o:p></o:p>
Atıf için künye
Dikbaş, N., Kızıler, Y. E., Cinisli, K. T., Uçar, S., & Canca, E. (2019). Molecular identification of Bacillus pumilus isolated from natural resources by 16S rRNA, chitinase enzyme activity and the industrial relevance of the related enzyme. ECOMB 2019: Eurasian Congress on Molecular Biotechnology, Trabzon, Türkiye, 19 - 21 Eylül 2019, Trabzon (ss. 20–21).
BibTeX
@inproceedings{dikbas2019-281556,
author = {Dikbaş, Neslihan and Kızıler, Yakup Emre and Cinisli, Kağan Tolga and Uçar, Sevda and Canca, Emre},
title = {Molecular identification of Bacillus pumilus isolated from natural resources by 16S rRNA, chitinase enzyme activity and the industrial relevance of the related enzyme},
booktitle = {ECOMB 2019: Eurasian Congress on Molecular Biotechnology, Trabzon, Türkiye, 19 - 21 Eylül 2019},
address = {Trabzon},
year = {2019},
pages = {20--21},
url = {https://akademik.atauni.edu.tr/yayinlar/281556-molecular-identification-of-bacillus-pumilus-isolated-from-natural-resources-by}
}