Enhanced Lipid Yield from Olive-Mill Wastewater by Yarrowia lipolytica NRRL YB-423

Lipid production from olive-mill wastewater (OMW) by Yarrowia lipolytica NRRL YB-423 was optimized (biomass concentration and lipid yield based on dry cell weight) using multi-response criteria based on the Taguchi orthogonal array. Sixteen experimental runs were performed using the L16 orthogonal array. Dilution rates of OMW (15, 30, 45, and 60%), Tween 80 (0, 0.2, 0.4, and 0.6%), sodium chloride (NaCl; 0, 1, 2, and 3%), and sterility were selected as factors. The significance of the parameters was determined using analysis of variance (ANOVA). The effects of all factors on the lipid yield were statistically significant (p<0.05). The results showed that sterility had a maximum contribution of 48.12% to lipid yield. The highest lipid yield (40.88 %) was achieved in sterile medium supplemented with 15% diluted OMW, 0.6% Tween 80, and 3% NaCl.