Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach

Razgonova, Mayya P.; Zinchenko, Yulia N.; Kozak, Darya K.; Kuznetsova, Victoria A.; Zakharenko, Alexander M.; Ercişli, Sezai; Golokhvast, Kirill S.

doi:10.3390/molecules27238228

Makale Tam Makale Uluslararası 2022 Açık erişimli

Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach

Mayya P. Razgonova, Yulia N. Zinchenko, Darya K. Kozak, Victoria A. Kuznetsova, Alexander M. Zakharenko, Sezai Ercişli, Kirill S. Golokhvast

MOLECULES Cilt 27 · Sayı 23 · 01.12.2022

DOI ile aç

Özet

© 2022 by the authors.In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.

Anahtar kelimeler

IDENTIFICATION ANTIOXIDANT ACTIVITY MS QUANTIFICATION L. flavonols SECONDARY METABOLITES POLYPHENOLS TANDEM MASS-SPECTROMETRY HPLC-DAD HPLC-MS/MS Cell walls HPLC-MS laser microscopy Glycine Willd COWPEA VIGNA-UNGUICULATA SEED COATS

Atıf için künye

Razgonova, M. P., Zinchenko, Y. N., Kozak, D. K., Kuznetsova, V. A., Zakharenko, A. M., Ercişli, S., & Golokhvast, K. S. (2022). Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach. MOLECULES, 27(23). https://doi.org/10.3390/molecules27238228

BibTeX

@article{razgonova2022-264881,
  author = {Razgonova, Mayya P. and Zinchenko, Yulia N. and Kozak, Darya K. and Kuznetsova, Victoria A. and Zakharenko, Alexander M. and Ercişli, Sezai and Golokhvast, Kirill S.},
  title = {Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach},
  journal = {MOLECULES},
  year = {2022},
  volume = {27},
  number = {23},
  issn = {1420-3049},
  doi = {10.3390/molecules27238228},
  url = {https://akademik.atauni.edu.tr/yayinlar/264881-autofluorescence-based-investigation-of-spatial-distribution-of-phenolic}
}