Genetic polymorphism in Kappa-casein gene detected by PCR-RFLP in cattle

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method has been used to detect polymorphism in Kappa-casein gene in cattle. A 351 bp Kappa-casein gene fragment was amplified and the PCR products were digested with four different restriction enzymes i.e. Alu I, Hind III, Hinf I and Taq I. All these enzymes were able to distinguish between Kappa-casein A and B types, however Hind III, Hinf I and Taq I were found more effective for kappa-casein variants than Alu I.