Genotyping of Brucella isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA)

Ozmen, Murat; Özgen, Ediz Kağan; Sayi, Orbay; Putur, Elif Karadeniz; Okumus, Biray; Yilmaz, Sibel Iba; Aslan, Mehtap Hulya; Ulucan, Mustafa; Yanmaz, Berna; Bagatir, Perihan Serifoglu; Turut, Nevin; Karahan, Sifa; Eroglu, Buket; Gulseren, Yildiz Oz; Kucukayan, Ugur; Nuhay, Cagatay; Eskiizmirliler, Seza; Aka, Erdem S.; Sarisahin, Asuman Soysal; Deniz, Ibrahim; Birben, Nurgul; Karakus, Kerem; Sen, Esra; Saytekin, Ahmet Murat; Akar, Kadir

doi:10.1016/j.cimid.2023.101981

Makale Tam Makale Uluslararası 2023

Genotyping of Brucella isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA)

Murat Ozmen, Ediz Kağan Özgen, Orbay Sayi, Elif Karadeniz Putur, Biray Okumus, Sibel Iba Yilmaz, Mehtap Hulya Aslan, Mustafa Ulucan, Berna Yanmaz, Perihan Serifoglu Bagatir, Nevin Turut, Sifa Karahan, Buket Eroglu, Yildiz Oz Gulseren, Ugur Kucukayan, Cagatay Nuhay, Seza Eskiizmirliler, Erdem S. Aka, Asuman Soysal Sarisahin, Ibrahim Deniz, Nurgul Birben, Kerem Karakus, Esra Sen, Ahmet Murat Saytekin, Kadir Akar

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES Cilt 96 · 01.05.2023

DOI ile aç

Özet

This study investigates country-wide genotype variations through the genotyping of Brucella strains isolated from domestic ruminants and humans. The Brucella spp. isolated from samples taken from animals and humans were first identified as B. abortus and B. melitensis by real-time PCR, and the MLVA-16 approach was then used for the genotyping of the identified isolates. For the study, 416 Brucella spp. were isolated from aborted fetus samples examined between 2018 and 2021, and 74 Brucella spp. from infected humans. Of the 74 human isolates analyzed, 1.3% were identified as B. abortus and 98.7% (73/74) as B. melitensis. The MLVA-16 typing method revealed 30 clonal groups for B. abortus and 37 clonal groups for B. melitensis from which the dominant genotypes and similarities with human isolates in Turkiye were determined.

Anahtar kelimeler

INFECTION BACTEREMIA BOVINE Türkiye molecular identification B/ Abortus MELITENSIS MLVA-16 Ruminant BIOVAR 3 OVINE

Atıf için künye

Ozmen, M., Özgen, E. K., Sayi, O., Putur, E. K., Okumus, B., Yilmaz, S. I., Aslan, M. H., Ulucan, M., Yanmaz, B., Bagatir, P. S., Turut, N., Karahan, S., Eroglu, B., Gulseren, Y. O., Kucukayan, U., Nuhay, C., Eskiizmirliler, S., Aka, E. S., Sarisahin, A. S., … Akar, K. (2023). Genotyping of Brucella isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA). COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 96. https://doi.org/10.1016/j.cimid.2023.101981

BibTeX

@article{ozmen2023-261691,
  author = {Ozmen, Murat and Özgen, Ediz Kağan and Sayi, Orbay and Putur, Elif Karadeniz and Okumus, Biray and Yilmaz, Sibel Iba and Aslan, Mehtap Hulya and Ulucan, Mustafa and Yanmaz, Berna and Bagatir, Perihan Serifoglu and Turut, Nevin and Karahan, Sifa and Eroglu, Buket and Gulseren, Yildiz Oz and Kucukayan, Ugur and Nuhay, Cagatay and Eskiizmirliler, Seza and Aka, Erdem S. and Sarisahin, Asuman Soysal and Deniz, Ibrahim and Birben, Nurgul and Karakus, Kerem and Sen, Esra and Saytekin, Ahmet Murat and Akar, Kadir},
  title = {Genotyping of Brucella isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA)},
  journal = {COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES},
  year = {2023},
  volume = {96},
  issn = {0147-9571},
  doi = {10.1016/j.cimid.2023.101981},
  url = {https://akademik.atauni.edu.tr/yayinlar/261691-genotyping-of-brucella-isolates-from-animals-and-humans-by-multiple-locus}
}