Effects of Gallic Acid on Nrf-2 and HO-1 Levels on ACR-Induced Kidney Damage in Rats

Acrylamide (ACR) is a chemical that is widely used in many areas and can cause damage

to many tissues and organs. It occurs as a result of heat treatment of foods containing

mainly carbohydrates and proteins. In this study, the effects of gallic acid (GA) on kidney

damage induced by ACR in rats were investigated. A total of 40 male Sprague Dawley

rats were used in our study and the rats were divided into five experimental groups which

Control, ACR, ACR+GA50, ACR+GA100 and GA100. In the experiment, ACR was

applied to the ACR groups at a dose of 50 mg/kg, while Gallic acid (GA) was applied to

the GA groups at doses of 50 and 100mg/kg. At the end of the 14-day experiment, the

rats were euthanized and their kidney tissues were removed. In order to determine

whether oxidative stress developed in kidney tissues taken from rats, the nuclear factor

erythroid 2–related factor 2 (Nrf-2) and heme oxygenase-1 (HO-1) levels were

determined by using the ELISA method. While both Nrf-2 and HO-1 levels decreased

significantly in the groups treated with ACR, there was an increase in the kidney tissues

of the treated rats with GA. As a result, in this study was determined that GA had a

protective effect against ACR-induced renal oxidative stress and this protective effect was

achieved especially with high doses of GA.