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Bildiri Poster Uluslararası 2023

Effects of Melatonin on Inflammatory Mediators iNOS, COX-2, and PGE2 in Testicular Toxicity Induced by Acrylamide in Rats

Emin Şengül, Merve Bolat, Samet Tekin

4 TH INTERNATIONAL CONGRESS ON ENGINEERING AND LIFE SCIENCE “Life in the Grip of Pandemic and War: Agriculture and Nature” ss. 241–247 · Comrat · 17.11.2023–19.11.2023 · 17.11.2023

Özet

Abstract: Acrylamide (ACR) is a chemical that can pose a serious carcinogenic risk and is formed during thermal

processes, such as the high-temperature cooking or frying of starchy foods. Exposure to products created through these

means causes damage to individuals. ACR exposure cause to damage in various tissues and organs. Additionally, the

observed gonadotoxic effects in experimental animals emphasize the potential harms of this substance. Melatonin (MEL)

is a versatile compound secreted by the pineal gland in vertebrates, with various effects and functions. It has been found

to play a significant role in regulating steroid hormone secretion in the male reproductive process. Therefore, this study

investigated the effect of MEL on ACR-induced testicular toxicity in rats. Rats were randomly divided into five groups:

The Control, ACR, MEL10+ACR, MEL20+ACR, and MEL20 group. In the ACR groups, ACR was administered at a

dose of 50 mg/kg, while in the MEL groups, MEL was administered at doses of 10 mg/kg and 20 mg/kg. The experiment

continued for 14 days, and it was concluded on the 15th day. At the end of the experiment, testicular tissues obtained

from the rats were placed in -80°C freezer. Testicular tissues removed from the freezer on the day of the experiment were

subjected to homogenization. The resulting supernatants were analyzed according to the ELISA protocol. It was observed

that exposure to ACR increased COX-2, iNOS, and PGE2 levels in the supernatants prepared from the tissues of rats

exposed to ACR. ACR exposure was found to cause testicular tissue damage in rats. The application of MEL along with

ACR significantly reduced the testicular toxicity induced by ACR. Melatonin exhibited anti-inflammatory effects by

reducing COX-2, iNOS, and PGE2 levels. However, we believe that conducting more detailed research at this level would

be appropriate.

Keywords: Acrylamide, Gonadotoxic, Inflammation, Testis, Rat

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler
  • SKA 11: Sürdürülebilir Şehir ve Yaşam Alanları

Atıf için künye

Şengül, E., Bolat, M., & Tekin, S. (2023). Effects of Melatonin on Inflammatory Mediators iNOS, COX-2, and PGE2 in Testicular Toxicity Induced by Acrylamide in Rats. 4 TH INTERNATIONAL CONGRESS ON ENGINEERING AND LIFE SCIENCE “Life in the Grip of Pandemic and War: Agriculture and Nature”, Comrat (ss. 241–247).

BibTeX 
@inproceedings{sengul2023-257685,
  author = {Şengül, Emin and Bolat, Merve and Tekin, Samet},
  title = {Effects of Melatonin on Inflammatory Mediators iNOS, COX-2, and PGE2 in Testicular Toxicity Induced by Acrylamide in Rats},
  booktitle = {4 TH INTERNATIONAL CONGRESS ON ENGINEERING AND LIFE SCIENCE “Life in the Grip of Pandemic and War: Agriculture and Nature”},
  address = {Comrat},
  year = {2023},
  pages = {241--247},
  url = {https://akademik.atauni.edu.tr/yayinlar/257685-effects-of-melatonin-on-inflammatory-mediators-inos-cox-2-and-pge2-in-testicular}
}