Biochemical and Pathological Effects on the Male Rat Hepatic Tissue After Exposure to 900mhz Electromagnetic Field During Adolescent Period.
Özet
The aim of this study was to employ biochemical analysis and
histopathological techniques to investigate the effects of the application of a 900-
megahertz electromagnetic field during adolescence on the rat liver. 24 male
Sprague Dawley rats aged 21 days were randomly divided into electromagnetic
field, sham and control groups. No procedure was performed on the control group
rats. Electromagnetic field group rats were exposed to a 900-megahertz
electromagnetic field in an electromagnetic field application cage (1 hours/day,
every day for 25 days). The sham group rats were placed in the same
electromagnetic field cage without exposure to electromagnetic field. At the end
of the experiments, the livers were removed. The livers were used for
histopathological evaluation (light, electron and immunofluorescence microscopy)
and biochemical analyses. Biochemical analyses revealed increased lipid
peroxidation and glutathione values while catalase and superoxide dismutase
values were decreased in electromagnetic field group. Electron microscopy evaluations identified necrotic hepatocytes with numerous cytoplasmic and
mitochondrial vacuoles in electromagnetic field group sections. We also observed
an enlarged endoplasmic reticulum and loss of mitochondrial matrix, in addition
to the presence of vacuoles. Also, loss of inner and outer mitochondrial membrane
integrity was observed. Light microscopy evaluations revealed sinusoidal
capillary dilatation and degeneration in hepatocytes with cytoplasmic swelling,
vacuolar degeneration and pyknotic nuclei in electromagnetic field group sections.
Deterioration in the integrity of the collagen fibers was also present around the
vena centralis. Additionally, electromagnetic field had no effect on body weight.
Therefore, we conclude that continuous 900-megahertz electromagnetic field
treatment may cause changes in oxidative stress biomarkers and the morphology
of the adolescent rat liver.
Atıf için künye
İKİNCİ KELEŞ, A., EROL, H. S., SAPMAZ, T., MERCANTEPE, T., KELEŞ, G., BİTERGE SÜT, B., ODACI, E., Halıcı, M. B., & POLAT, S. (2021). Biochemical and Pathological Effects on the Male Rat Hepatic Tissue After Exposure to 900mhz Electromagnetic Field During Adolescent Period. Communications Faculty of Sciences University of Ankara Series C Biology, 30(1), 25–46. https://dergipark.org.tr/en/pub/communc/issue/58411/764890
BibTeX
@article{ikincikeles2021-256746,
author = {İKİNCİ KELEŞ, AYŞE and EROL, HÜSEYİN SERKAN and SAPMAZ, TUĞÇE and MERCANTEPE, TOLGA and KELEŞ, GÖKHAN and BİTERGE SÜT, BURCU and ODACI, ERSAN and Halıcı, Mesut Bünyami and POLAT, SAİT},
title = {Biochemical and Pathological Effects on the Male Rat Hepatic Tissue After Exposure to 900mhz Electromagnetic Field During Adolescent Period.},
journal = {Communications Faculty of Sciences University of Ankara Series C Biology},
year = {2021},
volume = {30},
number = {1},
pages = {25--46},
issn = {1303-6025},
url = {https://akademik.atauni.edu.tr/yayinlar/256746-biochemical-and-pathological-effects-on-the-male-rat-hepatic-tissue-after}
}