Investigation of the effects of rutin against diabetic nephropathy in rats

Abstract

Diabetes Mellitus is a disease characterized by excessively high blood sugar levels causing complications

in many systems. Diabetes mellitus can lead to disorders in carbohydrate, fat and protein metabolism as well

as serious health problems such as neuropathy, retinopathy, nephropathy, peripheral vascular disease,

coronary artery disease and atherosclerosis. Diabetic hepatopathies are a common chronic complication

affecting approximately 30% of patients with diabetes. Rutin (quercetin rutinoside) is a glycoside of the

flavonoid quercetin. Rutin prevents platelet aggregation has anti-inflammatory effects and reduces circulating

fat and cholesterol. In this study, we aimed to determine the effects of rutin against nephropathy due to dibetes

mellitus. For this purpose, 40 female Sprague-Dawley rats were randomly divided into 4 groups as Control,

Diabetes, Diabetes+Metformin and Diabetes+Rutin50 Groups. At the end of the study, rats were sacrificed

and kidney tissue samples were taken. In the diabetes group, degeneration, necrosis, glomerular atrophy and

hyperemia were observed in the renal tubule epithelium due to hyperglycemia while these pathological

conditions were significantly reduced in the routine treatment group. In addition, immunohistochemical and

immunofluorescence analyses revealed that the expression levels of TIM and 8-OHdG which were at very

high levels due to diabetes,were decreased significantly. As a result of the examinations, it was determined

that rutin treatment reduced apoptosis and DNA damage by reducing oxidative damage in kidney tissues and

increased tissue resistance by strengthening immunity. This suggested that Rutin had alternative or

complementary effects in the treatment of diabetes. We believe that it will shed light on future studies.

Key words: Diabetes mellitus, Oxidative stress, Rutin, TIM