Investigation of Neurotoxicity Oxidative Stress and Oxidative DNA Damage in Cadmium-Induced Brain Injury in Rats
Özet
ABSTRACT
Cadmium, which is one of the toxic metals widely used in many areas of the world, is taken into
the body in different ways. In this study, the damage caused by cadmium in the brain and how
this damage affects the levels of glial fibrial acidic protein, 8-Oxo-2’-deoxyguanosine (8-OHdG),
neuronal nitric oxide synthase, Süperoksit dismutaz (SOD), Glutatyon (GSH), glutatyon peroksidaz
(GPX), inducible nitric oxide synthase, and malondialdehid (MDA) expression in the brain tissue
were investigated. A total of 16 male Wistar albino rats 200-220 g were used in the experimental
study. Rats were divided into 2 groups. A single cadmium i.p. dose of 0.025 mmol/kg was given
to rats in the cadmium group. Rat brain tissue samples were analyzed using biochemical analyses as well as histopathological and immunohistochemical methods. In the histopathological
examination of the brain tissues, normal histological structure was observed in the brain samples
belonging to the control group, while necrosis and degeneration of neurons in the brain, as well
as hyperemia in the parenchyma and meningeal vessels were observed in the cadmium group.
In immunohistochemical examinations, while glial fibrial acidic protein, 8-OHdG, and neuronal
nitric oxide synthase expression was not observed in the brain samples of control group, severe
expression of glial fibrial acidic protein, 8-OHdG, and neuronal nitric oxide synthase was observed
in the brain tissue of the group receiving cadmium. In the biochemical analyses performed, it was
observed that SOD, GSH, and GPx enzyme levels increased in cadmium groups, inducible nitric
oxide synthase and MDA enzyme levels were decreased. As a result of this study, it is thought that
markers’ expression levels are important in understanding the oxidative stress in pathogenesis of
cadmium toxicity and will provide a guide and important contributions to future studies.
Keywords: Biochemical, cadmium, histopathology, immunohistochemistry
Sürdürülebilir Kalkınma Amaçları
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SKA 3: Sağlıklı Bireyler
Atıf için künye
BOLAT, İ., Yıldırım, S., CEYLAN, N., Sağlam, Y. S., ÖRNEK, F. K., Bolat, M., & Kiliçlioğlu, M. (2023). Investigation of Neurotoxicity Oxidative Stress and Oxidative DNA Damage in Cadmium-Induced Brain Injury in Rats. Veterinary sciences and practices (Online), 18, 19–24. https://doi.org/10.5152/vetscipract.2023.222441
BibTeX
@article{bolat2023-255496,
author = {BOLAT, İSMAİL and Yıldırım, Serkan and CEYLAN, NESRİN and Sağlam, Yavuz Selim and ÖRNEK, Ferit Kansu and Bolat, Merve and Kiliçlioğlu, Metin},
title = {Investigation of Neurotoxicity Oxidative Stress and Oxidative DNA Damage in Cadmium-Induced Brain Injury in Rats},
journal = {Veterinary sciences and practices (Online)},
year = {2023},
volume = {18},
pages = {19--24},
issn = {2822-3608},
doi = {10.5152/vetscipract.2023.222441},
url = {https://akademik.atauni.edu.tr/yayinlar/255496-investigation-of-neurotoxicity-oxidative-stress-and-oxidative-dna-damage-in}
}