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Makale Tam Makale Uluslararası 2018

Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide

Liina KİNKAR, Teivi LAURİMAE, Gerardo Acosta-Jamett, Vanessa ANDRESİUK, İbrahim Balkaya, Adriano CASULLİ, Robin B. Gasser, Luis MİGUEL GONZALEZ, Karen L. Haag, Houria ZAİT, Malik Irshadullah, Abdul Jabbar, David J. Jenkins, Maria Teresa MANFREDİ, Hossein MIRHENDI, Selim M'rad, Mohammad Rostami-Nejad, Myriam Oudni-M'rad, Nora BEATRIZ PIERANGELI, Francisco Ponce-Gordo, Steffen REHBEİN, Mitra Sharbatkhori, Eshrat Beigom KIA, Sami Simsek, Silvia Viviana Soriano, Hein SPRONG, Viliam SNABEL, Gerald UMHANG, Antonio VARCASİA, Urmas SAARMA

INFECTION GENETICS AND EVOLUTION Cilt 64 · ss. 178–184 · 01.10.2018
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Özet

Cystic echinococcosis (CE), a zoonotic disease caused by tapeworms of the species complex Echinococcus granulosus sensu lato, represents a substantial global health and economic burden. Within this complex, E. granulosus sensu stricto (genotypes G1 and G3) is the most frequent causative agent of human CE. Currently, there is no fully reliable method for assigning samples to genotypes G1 and G3, as the commonly used mitochondrial coxl and nad1 genes are not sufficiently consistent for the identification and differentiation of these genotypes. Thus, a new genetic assay is required for the accurate assignment of G1 and G3. Here we use a large dataset of near-complete mtDNA sequences (n = 303) to reveal the extent of genetic variation of G1 and G3 on a broad geographical scale and to identify reliable informative positions for G1 and G3. Based on extensive sampling and sequencing data, we developed a new method, that is simple and cost-effective, to designate samples to genotypes G1 and G3. We found that the nad5 is the best gene in mtDNA to differentiate between G1 and G3, and developed new primers for the analysis. Our results also highlight problems related to the commonly used cox1 and nad1.

Anahtar kelimeler

taxonomy SEQUENCE MITOCHONDRIAL Cystic echinococcosis NUCLEAR TAPEWORM Echinococcus granulosus sensu stricto Genotype identification Mitochondrial markers The nad5 gene MOLECULAR-GENETIC CHARACTERIZATION CESTODA TAENIIDAE PHYLOGENETIC-RELATIONSHIPS CERVID STRAIN CANADENSIS

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

KİNKAR, L., LAURİMAE, T., Acosta-Jamett, G., ANDRESİUK, V., Balkaya, İ., CASULLİ, A., Gasser, R. B., MİGUEL GONZALEZ, L., Haag, K. L., ZAİT, H., Irshadullah, M., Jabbar, A., Jenkins, D. J., MANFREDİ, M. T., MIRHENDI, H., M'rad, S., Rostami-Nejad, M., Oudni-M'rad, M., BEATRIZ PIERANGELI, N., … SAARMA, U. (2018). Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide. INFECTION GENETICS AND EVOLUTION, 64, 178–184. https://doi.org/10.1016/j.meegid.2018.06.026

BibTeX 
@article{kinkar2018-255119,
  author = {KİNKAR, Liina and LAURİMAE, Teivi and Acosta-Jamett, Gerardo and ANDRESİUK, Vanessa and Balkaya, İbrahim and CASULLİ, Adriano and Gasser, Robin B. and MİGUEL GONZALEZ, Luis and Haag, Karen L. and ZAİT, Houria and Irshadullah, Malik and Jabbar, Abdul and Jenkins, David J. and MANFREDİ, Maria Teresa and MIRHENDI, Hossein and M'rad, Selim and Rostami-Nejad, Mohammad and Oudni-M'rad, Myriam and BEATRIZ PIERANGELI, Nora and Ponce-Gordo, Francisco and REHBEİN, Steffen and Sharbatkhori, Mitra and KIA, Eshrat Beigom and Simsek, Sami and Viviana Soriano, Silvia and SPRONG, Hein and SNABEL, Viliam and UMHANG, Gerald and VARCASİA, Antonio and SAARMA, Urmas},
  title = {Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide},
  journal = {INFECTION GENETICS AND EVOLUTION},
  year = {2018},
  volume = {64},
  pages = {178--184},
  issn = {1567-1348},
  doi = {10.1016/j.meegid.2018.06.026},
  url = {https://akademik.atauni.edu.tr/yayinlar/255119-distinguishing-echinococcus-granulosus-sensu-stricto-genotypes-g1-and-g3-with}
}