Bir kurtta (Canis lupus) Echinocccus granulosus sensu stricto ve Echinococcus multilocularis olgusu

Objectives: With this study, it was aimed to

investigate the presence of Echinococcus

species in a wolf.

Materials-Methods: The necropsy of the wolf which

was found dead in Erzurum's Yakutiye district was

carried out. The intestinal contents were examined

under a stereomicroscope with the sedimentation

and counting technique. Adult parasites were evaluated according to morphological criteria. Isolation

of DNA from adult parasites was executed. Echinococcus granulosus adults were subjected to Polymerase Chain Reaction (PCR) with JB3-JB4.5 primer pairs which amplify the cytochrome c oxidase subunit

1 (COI) gene. Echinococcus multilocularis adults

were subjected to PCR with Emnest/for and Emnest/rev primer pairs which amplify the 12S rRNA

gene region. The obtained amplicons were sent for

sequence analysis. The sequence data were edited,

aligned, and compared with reference isolates.

Then nucleotide sequences of E.multilocularis and

E.granulosus sensu stricto (s.s.) isolates were registered into the GenBank database.

Results: Echinococcus spp. were detected by sedimentation and counting technique. Species were

identified as E.granulosus and E.multilocularis according to morphological criteria. The result of

BLAST analysis of the adult E.granulosus sequences

was identified as G1 genotype (Accession no;

OK357541). Echinococcus multilocularis was confirmed by the sequence analysis of the obtained PCR

amplicons (Accession no; MT321279).

Conclusions: The study is presented the first report

of the presence of E.granulosus s.s and E.multilocularis in a wolf in Turkey. The detection of both adult

parasites in the same final host confirms that the

study result is supported that Erzurum is an endemic region for cystic and alveolar echinococcosis

thanks to presence of both Echinocccus adults in

one definitive host.