A Comparative Analysis of The Effects of Fertil Chip and Lazer Irradiation Techniques on Spermatological Parameters ın Post-Thaw Ram Semen

Aim: The aim of this

study was to compare the effects of Fertile Plus and laser applications on

spermatological parameters of ram semen following cryopreservation.<o:p></o:p>

Material and Method: In the study, four rams were used, and the semen samples were

divided into four groups, each consisting of four animals: Group 1:

frozen-thawed control semen; Group 2: frozen-thawed semen exposed to laser

treatment; Group 3: frozen-thawed semen processed through the Fertile Plus

chip; and Group 4: frozen-thawed semen exposed to laser treatment and

subsequently processed through the Fertile Plus chip.<o:p></o:p>

Results: In this study, the

effects of laser, microfluidic chip , and the laser+chip combination on

spermatological parameters of ram semen were investigated. Data obtained from

ten replicates demonstrated that laser and chip applications preserved plasma

membrane integrity, reduced the rate of abnormal spermatozoa, and enhanced

mitochondrial activity. The highest mitochondrial activity and the lowest

plasma membrane damage were observed in the laser+chip group. No significant

differences were detected among the groups in terms of DNA fragmantation. The

findings indicate that the combination of laser and microfluidic chip can be an

effective approach to improve the quality of ram semen.<o:p></o:p>

Conclusion:

Laser and microfluidic chip applications reduced plasma membrane damage,

decreased abnormal spermatozoa rate, and increased mitochondrial activity. The

best results were obtained in the laser+chip group. No significant differences

were found among the groups in terms of DNA fragmantation.<o:p></o:p>