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Effect of resveratrol on post-thaw motility, kinematics, structural parameters and antioxidant/oxidant status of Kamori buck spermatozoa

Tarique Hussain, Muhammad Hammad Fayyaz, Amjad Hameed, Syed Murtaz Hassan Andrabi, Rehana Kausar, Yasin Mubashir, Iqra Batool, Muhammad Shahzad, Ali Doğan Ömür

CRYOBIOLOGY Cilt 118 · ss. 1–9 · 01.03.2025
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Özet

Resveratrol is a polyphenol compound showing strong antioxidant properties. It is believed that semen cryopreservation causes significant sperm losses which eventually affects sperm quality. Improving antioxidant status

of semen may reduce this damage and enhance sperm fertilizing potential. This study investigated the resveratrol

treated freeze-thawing sperm motion, kinetics, structural parameters and antioxidant/oxidant status of Kamori

buck spermatozoa. Thirty-two ejaculates from 4 fertile Kamori bucks were processed in tris-citric-glyercol-egg

yolk (TCG-EY) based with varying concentrations of resveratrol (0, 10, 20, 40 and 50 μM). Over 75 % sperm

motility were pooled and frozen in liquid nitrogen. The results unveiled that adding resveratrol at 40 and 50 μM

concentration significantly enhanced (P < 0.05) total motility (progressive motility, rapid velocity, and average

path velocity, straight line velocity, curvilinear velocity, amplitude of lateral head displacement, beat cross

frequency, straightness and linearity in contrast with control and other groups. Supplementing resveratrol at 40

and 50 μM concentration significantly improved (P < 0.05) functional plasma membrane integrity, acrosome

integrity, whereas, all resveratrol groups had same significant (P < 0.05) effect on DNA integrity in response to

control group. The 40 and 50 μM resveratrol significantly promoted (P < 0.05) superoxide dismutase (SOD),

catalase (CAT), peroxidases (POD), and total antioxidant capacity (TAC) levels while significantly reducing (P <

0.05) the total oxidant status (TOS) and malondialdehyde (MDA) contents as compared to control and other

groups, respectively. The principal component analysis (PCA) exhibited samples were present in different

clusters except two groups which had partially overlapped. Using hierarchical clustering analysis, two clusters

were constructed showing the relationship within the treated groups. The results of spearman correlation coefficient showed that yellow color showed highly positive correlation while turquoise color exhibited highly

negative association between sperm variables. Overall, the results concluded that resveratrol at 50 μM performed

slightly better results than 40 μM in terms of improving sperm quality parameters.

Atıf için künye

Hussain, T., Fayyaz, M. H., Hameed, A., Andrabi, S. M. H., Kausar, R., Mubashir, Y., Batool, I., Shahzad, M., & Ömür, A. D. (2025). Effect of resveratrol on post-thaw motility, kinematics, structural parameters and antioxidant/oxidant status of Kamori buck spermatozoa. CRYOBIOLOGY, 118, 1–9. https://doi.org/10.1016/j.cryobiol.2025.105202

BibTeX 
@article{hussain2025-253313,
  author = {Hussain, Tarique and Fayyaz, Muhammad Hammad and Hameed, Amjad and Andrabi, Syed Murtaz Hassan and Kausar, Rehana and Mubashir, Yasin and Batool, Iqra and Shahzad, Muhammad and Ömür, Ali Doğan},
  title = {Effect of resveratrol on post-thaw motility, kinematics, structural parameters and antioxidant/oxidant status of Kamori buck spermatozoa},
  journal = {CRYOBIOLOGY},
  year = {2025},
  volume = {118},
  pages = {1--9},
  issn = {0011-2240},
  doi = {10.1016/j.cryobiol.2025.105202},
  url = {https://akademik.atauni.edu.tr/yayinlar/253313-effect-of-resveratrol-on-post-thaw-motility-kinematics-structural-parameters-and}
}