Atatürk Üniversitesi Atatürk Üniversitesi
Ara
Yayınlara dön
Makale Tam Makale Uluslararası 2024

Quercetin in semen extender improves frozen-thawed spermatozoa quality and in-vivo fertility in crossbred Kamori goats

Iqra Batool, Muhammad Hammad Fayyaz, Amjad Hameed, Syed Murtaza Hassan Andrabi, Rehana Kausar, Muhammad Shahzad, Yasin Mubashir, Ali Doğan Ömür, Ghulam Murtaza, Allah Ditta, Tarique Hussain

FRONTIERS IN VETERINARY SCIENCE Cilt 11 · Sayı 1 · Makale no 1 · ss. 1–9 · 01.04.2024
DOI ile aç

Özet

This study investigated the antioxidant effect of quercetin-treated semen on

frozen–thawed spermatozoa quality and in-vivo fertility in crossbred Kamori

goats. In total, 32 ejaculates from four fertile bucks were diluted in Tris-based

egg yolk extender with varying levels of quercetin (0, 1, 5, 10, and 15  μM).

Qualified semen samples were pooled and frozen in French straws. The

results revealed that the addition of quercetin in the semen extender increased

(p  <  0.05) frozen–thawed sperm total motility (TM), progressive motility (PM),

rapid velocity (RV), average path velocity (VAP), straight line velocity (VSL),

curvilinear velocity (VCL), and amplitude of lateral head (ALH) displacement in

contrast to the control group. Quercetin supplementation had no effect on beat

cross frequency (BCF), straightness (STR), and linearity (LIN) (p  >  0.05). Quercetin

showed significantly higher (p  <  0.05) plasma membrane and acrosome integrity

and viability (p  <  0.05) of spermatozoa in contrast to the control group. Quercetin

in the semen extender significantly increased (p  <  0.05) superoxide dismutase

(SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and total

antioxidant capacity (TAC) levels while reduced (p  <  0.05) the contents of total

oxidant status (TOS) and malondialdehyde (MDA), which were in contrast to

the control group. Ultrasound results revealed that 24 out of 30 (80%) goats

were found pregnant when semen was treated with 5  μM quercetin while the

control group showed 18 out of 30 (60%) animals were pregnant. Thus, the

study concluded that 5  μM quercetin-treated semen was found to be efficient,

showed increased antioxidant status, and reduced oxidant production, leading

to improved spermatozoa quality and in-vivo fertility in goats

Atıf için künye

Batool, I., Fayyaz, M. H., Hameed, A., Andrabi, S. M. H., Kausar, R., Shahzad, M., Mubashir, Y., Ömür, A. D., Murtaza, G., Ditta, A., & Hussain, T. (2024). Quercetin in semen extender improves frozen-thawed spermatozoa quality and in-vivo fertility in crossbred Kamori goats. FRONTIERS IN VETERINARY SCIENCE, 11(1), 1–9. https://doi.org/10.3389/fvets.2024.1385642

BibTeX 
@article{batool2024-253312,
  author = {Batool, Iqra and Fayyaz, Muhammad Hammad and Hameed, Amjad and Andrabi, Syed Murtaza Hassan and Kausar, Rehana and Shahzad, Muhammad and Mubashir, Yasin and Ömür, Ali Doğan and Murtaza, Ghulam and Ditta, Allah and Hussain, Tarique},
  title = {Quercetin in semen extender improves frozen-thawed spermatozoa quality and in-vivo fertility in crossbred Kamori goats},
  journal = {FRONTIERS IN VETERINARY SCIENCE},
  year = {2024},
  volume = {11},
  number = {1},
  pages = {1--9},
  issn = {2297-1769},
  doi = {10.3389/fvets.2024.1385642},
  url = {https://akademik.atauni.edu.tr/yayinlar/253312-quercetin-in-semen-extender-improves-frozen-thawed-spermatozoa-quality-and-in}
}