Cytotoxic activity of Cousinia birandiana Hub.-Mor. extract and fractions against several cancer cell lines

Cousinia birandiana is one of the species

of the Cousinia genus from the Asteraceae family. This species is

endemic to Turkey and there are few studies on the pharmacological effects and

phytochemical content. In the current study, the 70% ethanol extract and n-hexane,

ethyl acetate, n-butanol, and water fractions from this plant were investigated

for their cytotoxic activity against DU-145 (human brain metastatic prostate

cancer), HeLa (human cervix cancer) and MCF-7 (human estrogen positive breast

cancer) cell lines by the MTT method after 24 h of treatment. In this study, cisplatin

was used as a positive control, which has been widely used in the treatment of

a range of cancers. According to the results, all the tested extracts showed

cytotoxicity in a dose-dependent manner. However, the ethyl acetate fraction was

found to be the most active with 78.86±1.896 μg/mL, 78.02±2.173 μg/mL, and

44.28±3,31 μg/mL IC50 values against DU-145, HeLa, and MCF-7 cell

lines, respectively. In conclusion, it is the first cytotoxic

activity study with C. birandiana species, and our future studies

will focus on cytotoxicity tests on other cancerous and healthy cell lines.<o:p></o:p>