The activity of matrix metalloproteinase-2 and -9 in serum of pregnant and non-pregnant bitches
Özet
The aim of this study was to investigate, whether the activity of matrix metalloproteinase (MMP)-2 and -9 in the serum of pregnant and non-pregnant bitches differs significantly. For this purpose, 81 blood samples were taken from pregnant bitches at days 5-13, 15-21, 24-31, 34-40 and 41-50 after mating, and 51 samples from non-pregnant animals at corresponding times. Relative enzyme activity, calculated as the percentage of serum enzyme activity on enzyme activity in a control sample, was determined with a commercially available assay after activation of serum MMPs with p-aminophenylmercuric acetate (APMA). In addition, serum oestradiol (E-2) and progesterone (P-4) concentrations were measured with an enzymeimmunoassay (EIA). In the pregnant bitches, at days 5-13 and 15-21 after mating, the mean activity of both MMPs was significantly higher than in non-pregnant animals (28.5% vs 24.5% and 27.7% vs 22.6%; p < 0.01). Moreover, in the pregnant bitches, significant correlations were detected between the serum enzyme activity and the serum concentrations of E-2 (-0.900; p < 0.05) and P-4 (+0.667; p < 0.05).
Atıf için künye
Schafer-Somi, S., Aksoy, O., Patzl, M., Findik, M., Erunal-Maral, N., Beceriklisoy, H., Polat, B., & Aslan, S. (2005). The activity of matrix metalloproteinase-2 and -9 in serum of pregnant and non-pregnant bitches. Reproduction in Domestic Animals, 40(1), 46–50. https://doi.org/10.1111/j.1439-0531.2004.00552.x
BibTeX
@article{schafersomi2005-252844,
author = {Schafer-Somi, S and Aksoy, OA and Patzl, M and Findik, M and Erunal-Maral, N and Beceriklisoy, HB and Polat, Bülent and Aslan, S},
title = {The activity of matrix metalloproteinase-2 and -9 in serum of pregnant and non-pregnant bitches},
journal = {Reproduction in Domestic Animals},
year = {2005},
volume = {40},
number = {1},
pages = {46--50},
issn = {0936-6768},
doi = {10.1111/j.1439-0531.2004.00552.x},
url = {https://akademik.atauni.edu.tr/yayinlar/252844-the-activity-of-matrix-metalloproteinase-2-and-9-in-serum-of-pregnant-and-non}
}