The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests

Türkez, Hasan

doi:10.1016/j.etp.2010.03.004

Makale Tam Makale Uluslararası 2011

The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests

Hasan Türkez

Experimental and Toxicologic Pathology Cilt 63 · Sayı 5 · ss. 453–457 · 01.07.2011

DOI ile aç

Özet

Titanium dioxide (TiO2) is used in several commercial products such as cosmetics, sunscreen, toothpaste and pharmaceuticals. However, some recent investigations have revealed that titanium particles generate potential harmful effects on the environment and humans. Because of its strong antioxidant activity, ascorbic acid (AA) is admitted to act as an anti-mutagenic agent. The present study was undertaken to investigate the protective effect of AA against TiO2-induced genotoxicity. Sister chromatid exchange (SCE), micronucleus (MN) and the comet assays were used to assess TiO2-induced genotoxicity and to establish the protective effects of AA. There were significant increases (P < 0.05) in both SCE and MN frequencies of cultures treated with TiO2 as compared to controls. However, co-application of AA (4.87 and 9.73 mu M) and TiO2 resulted in decreases of SCE and MN rates as compared to the group treated with titanium alone. Besides, significant reductions of primary DNA damage (comet assay) were determined when the AA was added to the cell culture medium simultaneously with TiO2. In conclusion, the preventive role of AA in alleviating TiO2-induced DNA damage was indicated for the first time in the present study. (C) 2010 Elsevier GmbH. All rights reserved.

Anahtar kelimeler

oxidative stress CELLS GENOTOXICITY ASCORBIC-ACID IN-VIVO DNA-DAMAGE comet assay VITAMIN-E TITANIUM-DIOXIDE micronucleus sister chromatid exchanges PERIPHERAL-BLOOD LYMPHOCYTES sister chromatid exchange INDUCED GENOTOXICITY ALVEOLAR MACROPHAGES ULTRAFINE TIO2 PARTICLES

Atıf için künye

Türkez, H. (2011). The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests. Experimental and Toxicologic Pathology, 63(5), 453–457. https://doi.org/10.1016/j.etp.2010.03.004

BibTeX

@article{turkez2011-245013,
  author = {Türkez, Hasan},
  title = {The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests},
  journal = {Experimental and Toxicologic Pathology},
  year = {2011},
  volume = {63},
  number = {5},
  pages = {453--457},
  issn = {0940-2993},
  doi = {10.1016/j.etp.2010.03.004},
  url = {https://akademik.atauni.edu.tr/yayinlar/245013-the-role-of-ascorbic-acid-on-titanium-dioxide-induced-genetic-damage-assessed-by}
}