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Makale Tam Makale Uluslararası 1998

An enzymatic method for the determination of free fatty acids in serum/plasma

Ahmet Kızıltunç, Fatih Akcay

Clinical chemistry and laboratory medicine Cilt 36 · Sayı 2 · ss. 83–86 · 01.02.1998
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Özet

Estimation of serum free fatty acids (FFA) in serum based on the formation of inorganic phosphate has been simplified by eliminating complex stages. The principle of the present method is based on breakdown of pyrophosphate, formed by thioesterification of free fatty acids with ATP and CoA with the aid of acyl-CoA synthetase (EC 6.2.1.3) to inorganic phosphate. This is measured using the reaction with molybdate. The reaction equations are as follows:

Atıf için künye

Kızıltunç, A., & Akcay, F. (1998). An enzymatic method for the determination of free fatty acids in serum/plasma. Clinical chemistry and laboratory medicine, 36(2), 83–86. https://doi.org/10.1515/cclm.1998.015

BibTeX
@article{kiziltunc1998-235247,
  author = {Kızıltunç, Ahmet and Akcay, Fatih},
  title = {An enzymatic method for the determination of free fatty acids in serum/plasma},
  journal = {Clinical chemistry and laboratory medicine},
  year = {1998},
  volume = {36},
  number = {2},
  pages = {83--86},
  issn = {1434-6621},
  doi = {10.1515/cclm.1998.015},
  url = {https://akademik.atauni.edu.tr/yayinlar/235247-an-enzymatic-method-for-the-determination-of-free-fatty-acids-in-serumplasma}
}