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Makale Tam Makale Uluslararası 2016

The Impact of Resveratrol on Oxidative Stress Induced by Methotrexate in Rat Ileum Tissue: Evaluation of Biochemical and Histopathological Features and Analysis of Gene Expression

ADALET ÖZÇİÇEK, NİHAL ÇETİN, Ferda Keskin Cimen, LEVENT TÜMKAYA, İsmail MALKOÇ, Mine Gülaboğlu, OĞUZHAN YARALI, BAHADIR SÜLEYMAN

MEDICAL PRINCIPLES AND PRACTICE Cilt 25 · Sayı 2 · ss. 181–186 · 01.01.2016
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Özet

Objective: The aim of this study was to assess the impact of resveratrol (RST) on oxidative stress induced by methotrexate in rat ileum tissue. Materials and Methods: Twenty-four rats were divided into 4 groups with 6 in each group. Each rat was orally administered the following every day for 30 days: group 1 (MTXG), methotrexate (MTX; 5 mg/kg); group 2 (RMTXG), MTX (5 mg/kg) plus RST (25 mg/kg/day); group 3 (RSTG), RST alone (25 mg/kg/day), and group 4 (controls), distilled water. After the rats had been sacrified, the ilea were removed for the assessment of malondialdehyde (MDA), total glutathione (tGSH) and glutathione peroxidase (GSH-Px). Gene expression analyses for interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and myeloperoxidase (MPO) were also performed. Hematoxylin and eosin-stained paraffinembedded sections of the ileum were analyzed under a light microscope and the findings were recorded. Statistical analyses of the data were performed using one-way ANOVA. Results: The administration of MTX in group 1 yielded a higher level of MDA (8.33 +/- 2.5 mu mol/g protein, p < 0.001) and lower levels of tGSH (0.97 +/- 0.29 nmol/g protein) and GSH-Px (5.22 +/- 0.35 U/g protein, p < 0.001) compared to the other groups. MTX also increased IL-1 beta (40.33 +/- 5.43 gene expression levels), TNF-alpha (6.08 +/- 0.59) and MPO gene expression (9 +/- 1.41) in group 1 compared to the controls (11.33 +/- 2.07, 2.15 +/- 0.33 and 3.43 +/- 0.48, respectively, p < 0.001). The impact of RST on IL-1 beta, TNF-alpha and MPO gene expression induced by MTX was observed as a reversal of these findings (p < 0.05). Severe inflammation, damage to the villus epithelium and crypt necrosis was observed histopathologically in the MTXG group, whereas only mild inflammation was seen in the RMTXG group. Conclusion: In this study, ileal damage caused by MTX was inhibited by RST. (C) 2015 S. Karger AG, Basel

Anahtar kelimeler

oxidative stress NITRIC-OXIDE DAMAGE EFFICACY antioxidant MODEL GENE-EXPRESSION INJURY resveratrol Rat Ileum tissue TUMOR-NECROSIS-FACTOR MUCOSITIS

Atıf için künye

ÖZÇİÇEK, A., ÇETİN, N., Cimen, F. K., TÜMKAYA, L., MALKOÇ, İ., Gülaboğlu, M., YARALI, O., & SÜLEYMAN, B. (2016). The Impact of Resveratrol on Oxidative Stress Induced by Methotrexate in Rat Ileum Tissue: Evaluation of Biochemical and Histopathological Features and Analysis of Gene Expression. MEDICAL PRINCIPLES AND PRACTICE, 25(2), 181–186. https://doi.org/10.1159/000442020

BibTeX 
@article{ozcicek2016-23381,
  author = {ÖZÇİÇEK, ADALET and ÇETİN, NİHAL and Cimen, Ferda Keskin and TÜMKAYA, LEVENT and MALKOÇ, İsmail and Gülaboğlu, Mine and YARALI, OĞUZHAN and SÜLEYMAN, BAHADIR},
  title = {The Impact of Resveratrol on Oxidative Stress Induced by Methotrexate in Rat Ileum Tissue: Evaluation of Biochemical and Histopathological Features and Analysis of Gene Expression},
  journal = {MEDICAL PRINCIPLES AND PRACTICE},
  year = {2016},
  volume = {25},
  number = {2},
  pages = {181--186},
  issn = {1011-7571},
  doi = {10.1159/000442020},
  url = {https://akademik.atauni.edu.tr/yayinlar/23381-the-impact-of-resveratrol-on-oxidative-stress-induced-by-methotrexate-in-rat}
}