The investigation of possible roles of central 5-HT7 receptors in antipyretic effect mechanism of paracetamol in LPS-induced hyperthermia model of mice

KÖSE, Duygu; Çadırcı, Elif; Halıcı, Zekai; Sirin, Busra; DİNCER, BÜŞRA

doi:10.1007/s10787-019-00617-1

Makale Tam Makale Uluslararası 2019

The investigation of possible roles of central 5-HT7 receptors in antipyretic effect mechanism of paracetamol in LPS-induced hyperthermia model of mice

Duygu KÖSE, Elif Çadırcı, Zekai Halıcı, Busra Sirin, BÜŞRA DİNCER

Inflammopharmacology Cilt 27 · Sayı 6 · ss. 1169–1178 · 01.12.2019

DOI ile aç

Özet

Aim This study aimed to investigate the role of the 5-HT7 receptor in fever mechanisms and its possible effect on the antipyretic mechanism of paracetamol. Materials and methods The study consisted of eight experimental groups and one control group. Group I: healthy, II: LPS, III: LPS + PARA, IV: LPS + AGO, V: LPS + ANTA, VI: LPS + AGO + ANTA, VII: LPS + AGO + PARA, VIII: LPS + ANTA + PARA, and IX: LPS + AGO + ANTA + PARA. Rectal temperatures were measured with a rectal thermometer. At the end of the experiment, tissues were examined molecularly. Real-time PCR mRNA expression analyses were performed for the 5-HT7 receptor, IL-6, and TNF-alpha in hypothalamus tissue. Results The mean differences in rectal temperature increased in the LPS, LPS + ANTA, and LPS + AGO + ANTA groups when compared to the healthy group and decreased in the LPS + PARA, LPS + AGO, LPS + AGO + PARA, and LPS + AGO + ANTA + PARA groups when compared to the healthy group. The IL-6 and TNF-alpha mRNA expression increased in the LPS, LPS + ANTA, and LPS + AGO + ANTA groups when compared to the healthy group in the 2nd and 4th hours. The IL-6 and TNF-alpha expression decreased in the LPS + PARA, LPS + AGO, LPS + AGO + PARA, and LPS + AGO + ANTA + PARA groups when compared to the LPS group in the 2nd and 4th hours. The 5-HT7 receptor mRNA expression increased in the LPS group when compared to the healthy group in the 2nd hour. The 5-HT7 receptor mRNA expression decreased in the LPS + AGO and LPS + AGO + PARA groups when compared to the LPS group in the 2nd hour. The 5-HT7 receptor mRNA expression increased the in LPS + ANTA and LPS + ANTA + PARA groups when compared to the LPS group in the 2nd hour. Conclusion The 5-HT7 receptor is a potential defense mechanism in stopping fever and the antipyretic property of paracetamol is not due to the 5-HT7 receptor.

Anahtar kelimeler

SYSTEM INFLAMMATION LIPOPOLYSACCHARIDE ACETAMINOPHEN BINDING 5-HT7 receptor paracetamol TNF- LPS TARGET RESPONSES Hypothermia fever Hypothalamus FEBRILE

Atıf için künye

KÖSE, D., Çadırcı, E., Halıcı, Z., Sirin, B., & DİNCER, B. (2019). The investigation of possible roles of central 5-HT7 receptors in antipyretic effect mechanism of paracetamol in LPS-induced hyperthermia model of mice. Inflammopharmacology, 27(6), 1169–1178. https://doi.org/10.1007/s10787-019-00617-1

BibTeX

@article{kose2019-231974,
  author = {KÖSE, Duygu and Çadırcı, Elif and Halıcı, Zekai and Sirin, Busra and DİNCER, BÜŞRA},
  title = {The investigation of possible roles of central 5-HT7 receptors in antipyretic effect mechanism of paracetamol in LPS-induced hyperthermia model of mice},
  journal = {Inflammopharmacology},
  year = {2019},
  volume = {27},
  number = {6},
  pages = {1169--1178},
  issn = {0925-4692},
  doi = {10.1007/s10787-019-00617-1},
  url = {https://akademik.atauni.edu.tr/yayinlar/231974-the-investigation-of-possible-roles-of-central-5-ht7-receptors-in-antipyretic}
}