Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats

Background and Objective: Exposure of the brain to Ischemia-Reperfusion (I/R) may cause tissue damage through oxidative stress. Thiamine pyrophosphate (TPP), which has a protective effect against oxidative stress, is the active metabolite of vitamin B-1. In this study, the protective effect of TPP against possible I/R damage of brain tissue was investigated. Materials and Methods: Thirty rats were randomly divided into BIR, TIR and HG groups consisting of ten rats. In the TIR group, 20 mg kgG1 TPP was injected intraperitoneally (ip). After 1 hr, clips were placed in the common carotid arteries of the BIR and TIR groups under anesthesia. Brain tissue was subjected to ischemia for 10 min. Afterward, the clips were opened and 3 hrs of reperfusion was achieved. In the HG group, only subcutaneous incisions were made and closed. Then, the brain tissues removed by euthanasia were biochemically analyzed. Results: While, I/R increased malondialdehyde (MDA), myeloperoxidase (MPO), Tumor Necrosis Factor-alpha (TNF-alpha), Interleukin-1 beta (IL-beta) and 8-Hydroxyguanine (8-OHGua) levels in brain tissues, total caused a decrease in glutathione (tGSH), glutathione peroxidase (GPO) and glutathione reductase (GSHRd) levels (p<0.001). The TPP applied before I/R significantly prevented these changes (p<0.05). Conclusion: The results of biochemical tests suggested that TPP may be beneficial in preventing possible brain damage due to I/R.