Effects of nicotine and vitamin E on glucose 6-phosphate dehydrogenase activity in some rat tissues in vivo and in vitro

Gumustekın, K; Cıftcı, M; Coban, A; Altıkat, S; Aktas, Osman; Gül, Mustafa; Tımur, Handan; Dane, S

doi:10.1080/14756360500277384

Makale Tam Makale Uluslararası 2005 Açık erişimli

Effects of nicotine and vitamin E on glucose 6-phosphate dehydrogenase activity in some rat tissues in vivo and in vitro

K Gumustekın, M Cıftcı, A Coban, S Altıkat, Osman Aktas, Mustafa Gül, Handan Tımur, S Dane

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Sayı 5 · ss. 497–502 · 01.10.2005

DOI ile aç

Özet

Effects of nicotine, and nicotine + vitamin E on glucose 6-phosphate dehydrogenase (G-6PD) activity in rat muscle, heart, lungs, testicle, kidney, stomach, brain and liver were investigated in vivo and in vitro on partially purified homogenates. Supplementation period was 3 weeks (n = 8 rats per group): nicotine [0.5 mg/kg/day, intraperitoneal (ip)].- nicotine + vitamin E [75 mg/kg/day, intragastric (ig)]; and control group (receiving only vehicle). The results showed that nicotine (0.5 mg/kg, ip) inhibited G-6PD activity in the lungs, testicle, kidney, stomach and brain by 12.5% (p < 0.001), 48% (p < 0.001), 20.8% (p < 0.001), 13% (p < 0.001) and 23.35% (p < 0.001) respectively, and nicotine had no effects on the muscle, heart and liver G6PD activity. Also, nicotine + vitamin E inhibited G-6PD activity in the testicle, brain, and liver by 32.5% (p < 0.001), 21.5% (p < 0.001), and 16.5% (p < 0.001) respectively, and nicotine + vitamin E activated the muscle, and stomach G-6PD activity by 36% (p < 0.05), and 20% (p < 0.001) respectively. In addition, nicotine + vitamin E did not have any effects on the heart, lungs, and kidney G-6PD activity. In addition, in vitro studies were also carried out to elucidate the effects of nicotine and vitamin E on G-6PD activity, which correlated well with in vivo experimental results in lungs, testicles, kidney, stomach, brain and liver tissues. These results show that vitamin E administration generally restores the inactivation of G-6PD activity due to nicotine administration in various rat tissues in vivo, and also in vitro.

Anahtar kelimeler

carbonic anhydrase INHIBITION GENE-EXPRESSION NICOTINE BRAIN VITAMIN-E INDUCED OXIDATIVE STRESS GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ENZYME-ACTIVITIES INACTIVATION HUMAN-ERYTHROCYTES rat tissues cotinine SHEEP LENS GLUCOSE-6-PHOSPHATE-DEHYDROGENASE SEROTONIN SYNTHESIS RHAMNOIDES-L.

Sürdürülebilir Kalkınma Amaçları

SKA 3: Sağlıklı Bireyler

Atıf için künye

Gumustekın, K., Cıftcı, M., Coban, A., Altıkat, S., Aktas, O., Gül, M., Tımur, H., & Dane, S. (2005). Effects of nicotine and vitamin E on glucose 6-phosphate dehydrogenase activity in some rat tissues in vivo and in vitro. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY(5), 497–502. https://doi.org/10.1080/14756360500277384

BibTeX

@article{gumustekin2005-224379,
  author = {Gumustekın, K and Cıftcı, M and Coban, A and Altıkat, S and Aktas, Osman and Gül, Mustafa and Tımur, Handan and Dane, S},
  title = {Effects of nicotine and vitamin E on glucose 6-phosphate dehydrogenase activity in some rat tissues in vivo and in vitro},
  journal = {JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY},
  year = {2005},
  number = {5},
  pages = {497--502},
  issn = {1475-6366},
  doi = {10.1080/14756360500277384},
  url = {https://akademik.atauni.edu.tr/yayinlar/224379-effects-of-nicotine-and-vitamin-e-on-glucose-6-phosphate-dehydrogenase-activity}
}