Development and Validation of A Reversed Phase-HPLC-DAD Method for Determination of Bisphenol-A in Artificial Saliva

Bisphenol-A is one of estrogenic xenobiotics present as an impurity in dental sealants and composites based on dimethacrylate monomers. In this Study, bisphenol-A in artificial saliva was analyzed by simple, accurate, precise and sensitive reversed phase HPLC-DAD method requiring simple sample preparation. Bisphenol-A separated by the reversed-phase C(18) column using simple (lie mobile phase containing isocratic mixture of acetonitrile-Milli-Q grade water (50:50 v/v) at flow rate of 1 mL min(-1). Linearity has been obtained in the range of 0.5-30 mu g mL(-1), whereas limit of detection and limit of quantification are 0.01 and 0.02 mu g mL(-1). respectively. Both intra day and inter day precision showed acceptable RSD values were lower than <6.3% and <9.5%, respectively. The recovery of developed method was also between 96.8-105.9%. It was suggested that proposed method has been successfully applied in routine analysis of bisphenol-A in saliva after dental treatment.