Development and Validation of HPLC-UV Method for Determination of Meloxicam in Tablet Dosage Formulation

Objective: The development and validation of a novel, simple, and quick high-performance liquid

chromatography–ultraviolet detection (HPLC-UV) technique for measuring meloxicam in pharmaceutical formulations was made.

Methods: The technique parameters were tuned to be 0.8 mL/min flow rate, variable column

temperature, 290 nm wavelength, 10 µL injection volume, and a mobile phase combination of

water (with 0.6% triflour acetic acid—pH:2.6) and methanol (30 : 70 v/v) to carry out this study. In

this study, valsartan was used as ınternal standard (IS).

Results: Specificity, the limit of quantitation (LOQ), linearity, accuracy, precision, stability, recovery, and ruggedness were all tested. The technique was linear between 1.0 µg/mL and 50 µg/mL,

with precision (relative standard d (RSD) %) and accuracy (relative error %) of less than 3.9% and

0.7%, respectively. The LOQ and LOD values of method were 1.00 and 0.25 µg/mL, respectively.

Analytical recovery from pharmaceutical preparations was performed according to the standard

addition method, and the average analytical recovery value was determined as 100.4%. The developed and validated HPLC-UV method was successfully applied to 4 commercial tablet dosage

formulations obtained from a local pharmacy store in Turkey (Zeloxim, Melox, Meksun, Exen).

Conclusion: It has been concluded that the developed HPLC-UV method is sensitive, accurate,

and precise and can be successfully applied in quality control studies in the pharmaceutical

industry.