Analysis of Atenolol in Rabbit Plasma by HPLC Method

The goal of this research is to develop a high-performance liquid chromatography method for analyzing atenolol levels in rabbit plasma and apply this method to the pharmacokinetic study. The liquid-liquid extraction technique was used to prepare blood samples from rabbits. Separation of atenolol was achieved on an $Ace C_{18}$ column. The method’s calibration curve was plotted between 5 and 250 ng/mL. The accuracy results were better than 2.97% and the precision results were less than 6.30% in rabbit plasma for atenolol. The method had recovery values >95.8% for all samples in rabbit plasma. In addition, the validated method was used to study atenolol pharmacokinetics in rabbits. The maximum atenolol plasma concentration is 240.1 ± 33.41 ng/mL. The duration to attain the greatest atenolol concentration and the area under the curve from$(AUC_{0-12 h})$were 3.0 ± 0.64 h and 1184.1 ± 235.13 ng/mL h, respectively.