Development and Validation of HPLC Method for the Determination of Flurbiprofen in Pharmaceutical Preparations

In this study, a new and rapid high-performance liquid chromatography (HPLC) method was developed for the determination of flurbiprofen in pure and pharmaceutical preparations. The method was developed on the Ace C18 column using a mobile phase of acetonitrile-0.05 M potassium dihydrogen phosphate solution (60:40, v/v) adjusted to pH 3.5 with phosphoric acid. The eluent was monitored by UV detection at 254 nm. The analysis was performed in less than 6 min with a flow rate of 1.0 mL min-1. Calibration curve was linear over the concentration range of 0.105.0 μg mL-1. Intra- and inter-day precision values for flurbiprofen were less than 4.56, and accuracy (relative error) was better than 4.00%. The mean recovery of flurbiprofen was 99.8% for pharmaceutical preparations. The limits of detection (LOD) and quantification (LOQ) were 0.03 and 0.10 μg mL-1, respectively. Also, the method was applied for the quality control of two commercial flurbiprofen dosage forms to quantify the drug and to check the formulation content uniformity.