Preparation and characterization of bFGF and BSA loaded microspheres

Çetin, Meltem; ÇAPAN, YILMAZ; VURAL, İMRAN; DOĞAN, AYŞE LALE; GÜÇ, DİCLE; Hıncal, Atilla; Wehrl, Pascal; DALKARA, TURGAY

doi:10.1016/s1773-2247(05)50067-4

Makale Tam Makale Uluslararası 2005

Preparation and characterization of bFGF and BSA loaded microspheres

Meltem Çetin, YILMAZ ÇAPAN, İMRAN VURAL, AYŞE LALE DOĞAN, DİCLE GÜÇ, Atilla Hıncal, Pascal Wehrl, TURGAY DALKARA

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY Cilt 15 · Sayı 5 · ss. 371–375 · 01.01.2005

DOI ile aç

Özet

This article describes the preparation and characterization of BSA and bFGF-loaded PLGA microspheres and assesses the effect of entrapment procedure and in vitro release conditions. In the experiments, changing the PVA concentration from 3 to 5% (w/v) in the pre-emulsion innerphase led to a change in particle size and encapsulation efficiency of BSA-loaded and bFGF-loaded PLGA microspheres from 1 to 0.7 mu m and from 73 to 65%, respectively. The in vitro release of the bFGF from PLGA microspheres was much lower than BSA-loaded PLGA microspheres. For all of the formulations, BSA and bFGF retained integrity after the microencapsulation process as evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, the cell proliferation-stimulating activity of bFGF was evaluated by using BALB/c 3T3 cells and it was shown that the bioactivity of bFGF was increased by adding heparin to the release medium.

Anahtar kelimeler

RELEASE ischemia PROTEIN nanoparticles PLGA bFGF SDS-PAGE FIBROBLAST-GROWTH-FACTOR BLOOD-BRAIN-BARRIER MICROSPHERES BSA POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES DELIVERY-SYSTEM NANOSPHERES GLYCOLIDE

Atıf için künye

Çetin, M., ÇAPAN, Y., VURAL, İ., DOĞAN, A. L., GÜÇ, D., Hıncal, A., Wehrl, P., & DALKARA, T. (2005). Preparation and characterization of bFGF and BSA loaded microspheres. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 15(5), 371–375. https://doi.org/10.1016/s1773-2247%2805%2950067-4

BibTeX

@article{cetin2005-21532,
  author = {Çetin, Meltem and ÇAPAN, YILMAZ and VURAL, İMRAN and DOĞAN, AYŞE LALE and GÜÇ, DİCLE and Hıncal, Atilla and Wehrl, Pascal and DALKARA, TURGAY},
  title = {Preparation and characterization of bFGF and BSA loaded microspheres},
  journal = {JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY},
  year = {2005},
  volume = {15},
  number = {5},
  pages = {371--375},
  issn = {1773-2247},
  doi = {10.1016/s1773-2247(05)50067-4},
  url = {https://akademik.atauni.edu.tr/yayinlar/21532-preparation-and-characterization-of-bfgf-and-bsa-loaded-microspheres}
}