Preparation and Characterization of Salmon Calcitonin-biotin Conjugates
Özet
This study was performed to prepare and characterize the biotinylated Salmon calcitonin (sCT) for oral delivery and evaluate the hypocalcemic effect of biotinylated-sCTs in rats. Biotinylated sCTs was characterized by using high performance liquid chromatography ( HPLC) and MALDITOF-MS. The effect of biotinylation on permeability across Caco-2 cell monolayers was examined. Their hypocalcemic effect was determined in rats. Mono- and di-bio-sCTs were separated by reverse phase HPLC. The molecular weights of mono-bio-sCT and di-bio-sCT were determined to be 3,660.5 and 3,900.2 Da, respectively. The permeability of biotinylated-sCTs across Caco-2 cell monolayers was observed with a significant enhancement compared with sCT. Intrajejunal (ij) administration of mono-bio-sCT and di-bios-CT resulted in sustained reduction in serum calcium levels, with a maximum reduction (% max(d)) of 21.6% and 30% after 4 h and 6 h of application, respectively. The biotin conjugation of sCT may be a promising strategy for increasing the oral bioavailability of sCT and achieving sustained calcium-lowering effects.
Anahtar kelimeler
Atıf için künye
Çetin, M., Youn, Y. S., Çapan, Y., & Lee, K. C. (2008). Preparation and Characterization of Salmon Calcitonin-biotin Conjugates. AAPS PHARMSCITECH, 9(4), 1191–1197. https://doi.org/10.1208/s12249-008-9165-2
BibTeX
@article{cetin2008-21531,
author = {Çetin, Meltem and Youn, Yu Seok and Çapan, Yılmaz and Lee, Kang Choon},
title = {Preparation and Characterization of Salmon Calcitonin-biotin Conjugates},
journal = {AAPS PHARMSCITECH},
year = {2008},
volume = {9},
number = {4},
pages = {1191--1197},
issn = {1530-9932},
doi = {10.1208/s12249-008-9165-2},
url = {https://akademik.atauni.edu.tr/yayinlar/21531-preparation-and-characterization-of-salmon-calcitonin-biotin-conjugates}
}