Anti-inflammatory effectiveness of daidzein in experimental knee osteoarthritis- induced with monosodium iodoacetate in rats

Osteoarthritis (OA) is a chronic and degenerative

disease that characterized by imbalance between the

events of making and destroying the periarticular bone,

degeneration of articular cartilage. It mostly occurs in

the knee joint. Daidzein (DZ) is a kind of

phytoestrogens of isoflovane group commonly that

found in many plants. The aim of this study was to

investigate the effects of DZ on the inflammatory

markers that has an important role in the pathogenesis

of OA. The protocol of this study was confirmed by

Local Ethics Committee of Laboratory Animals

Experiments of Ataturk University, Erzurum, Turkey. In

this study; Experimental knee osteoarthritis was formed

by monoiodoacete by intraarticular (ia) injection.

Twelve weeks age rats divided into 7 groups (Control

(untreatment), OA+saline, OA+DZ oral, OA+DZ ia,

OA+hyaluronic acid (HA) ia, OA+DZ oral+HA ia and

OA+DZ+HA ia. In oral groups, DZ was administered

with gavage twice daily for 21 days. In ia groups, DZ,

HA and saline were injected ia at 1st,7th, 14th and 21th

day of the experiment. At the end of the experiment

blood samples were taken for evaluating serum TNF-α

and IL-1β inflammatory markers with ELISA kits.

Results showed that serum TNF-α and IL-1β levels

decreased in the treatment groups compared to the

OA+saline group. But, OA+DZ oral+HA ia group was

only statistically significant decreased than OA+saline

group (p<0.05). It has been demonstrated that DZ has

anti inflammatory effect. At the same time, the results

of the study show that DZ alone and/or in combination

with HA may be useful in the treatment of knee OA.