Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles
Özet
The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.
Anahtar kelimeler
Atıf için künye
Çetin, M., Aktas, Y. E., Vural, I., Capan, Y., Dogan, L., Duman, M., & Dalkara, T. (2007). Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles. DRUG DELIVERY, 14(8), 525–529. https://doi.org/10.1080/10717540701606483
BibTeX
@article{cetin2007-21516,
author = {Çetin, Meltem and Aktas, Yunus Emre and Vural, I and Capan, Y and Dogan, La and Duman, M and Dalkara, T},
title = {Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles},
journal = {DRUG DELIVERY},
year = {2007},
volume = {14},
number = {8},
pages = {525--529},
issn = {1071-7544},
doi = {10.1080/10717540701606483},
url = {https://akademik.atauni.edu.tr/yayinlar/21516-preparation-and-in-vitro-evaluation-of-bfgf-loaded-chitosan-nanoparticles}
}