A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching
Özet
© 2022 The Author(s)Balancing the risks and benefits of organophosphate pesticides (OPs) on human and environmental health relies partly on their accurate measurement. A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs (triazophos, parathion, and chlorpyrifos) in apples, turnips, cabbages, and rice. Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs. DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification. The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore. The resulting fluorescence signal enables multiplexed quantification of triazophos, parathion, and chlorpyrifos residues over the concentration range of 0.01–25, 0.01–50, and 0.1–50 ng/mL with limits of detection of 0.014, 0.011, and 0.126 ng/mL, respectively. The mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%–17.6%, which correlate well with results obtained by LC-MS/MS. The proposed bio-barcode immunoassay is stable, reproducible and reliable, and is able to detect low residual levels of multi-residue OPs in agricultural products.
Anahtar kelimeler
Sürdürülebilir Kalkınma Amaçları
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SKA 3: Sağlıklı Bireyler
Atıf için künye
Xu, L., Zhang, X., Hassıbelnaby, A. M. A., Wang, Y., Cao, Z., Jia, H., Salvador, J., Hacımüftüoğlu, A., Cui, X., Zhang, Y., Wang, K., She, Y., Jin, F., Zheng, L., Pujia, B., Wang, J., Jin, M., & Hammock, B. D. (2022). A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching. Journal of Pharmaceutical Analysis, 12(4), 637–644. https://doi.org/10.1016/j.jpha.2022.05.004
BibTeX
@article{xu2022-210050,
author = {Xu, Lingyuan and Zhang, Xiuyuan and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Wang, Yuanshang and Cao, Zhen and Jia, Huiyan and Salvador, J.-Pablo and Hacımüftüoğlu, Ahmet and Cui, Xueyan and Zhang, Yudan and Wang, Kun and She, Yongxin and Jin, Fen and Zheng, Lufei and Pujia, Baima and Wang, Jing and Jin, Maojun and Hammock, Bruce D.},
title = {A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching},
journal = {Journal of Pharmaceutical Analysis},
year = {2022},
volume = {12},
number = {4},
pages = {637--644},
issn = {2095-1779},
doi = {10.1016/j.jpha.2022.05.004},
url = {https://akademik.atauni.edu.tr/yayinlar/210050-a-highly-sensitive-bio-barcode-immunoassay-for-multi-residue-detection-of}
}