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Simple and sensitive detection of triazophos pesticide by using quantum dots nanobeads based on immunoassay

Yun Liao, Xueyan Cui, Ge Chen, Yuanshang Wang, Guoxin Qin, Mingjie Li, Xiuyuan Zhang, Yudan Zhang, Chan Zhang, Pengfei Du, Feiyan Yan, Abdelaty Mostafa Abdelaty Hassıbelnaby, Jing Wang, Chenghui Zhang, Maojun Jin

FOOD AND AGRICULTURAL IMMUNOLOGY Cilt 30 · Sayı 1 · ss. 522–532 · 01.01.2019
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Özet

A rapid, ultra-sensitive and simple fluorescence immunoassay (FLISA) on CdSe/ZnS Quantum dots (QDs) for recognition pesticide triazophos have been established. In this assay, the immunoassay uses (CdSe/ZnS) Quantum dots (QDs) as a probe that carries monoclonal antibody (mAb). The free triazophos and OVA-haptens were competed for binding the mAb on the surface of the QDs. Therefore, the concentration of TRIAZ can get by calculation of the detection of fluorescence value. The assay has a linear response in the 10 ng L-1-25 mu g L-1 TRIAZ concentration range, and limit of detection (IC10) was 0.508 ng L-1. The recovery rate in the case of spiked samples ranges from 82.6% to 96.6%, and the RSD is <20%. FLISA can accurately and sensitively screen out triazophos in representative actual samples that contain trace levels of pesticides, it can play a prominent part in agricultural product and environmental detection.

Anahtar kelimeler

Fluorescence nanoparticles silver QUANTUM DOTS Residue Immunoassay antibody

Atıf için künye

Liao, Y., Cui, X., Chen, G., Wang, Y., Qin, G., Li, M., Zhang, X., Zhang, Y., Zhang, C., Du, P., Yan, F., Hassıbelnaby, A. M. A., Wang, J., Zhang, C., & Jin, M. (2019). Simple and sensitive detection of triazophos pesticide by using quantum dots nanobeads based on immunoassay. FOOD AND AGRICULTURAL IMMUNOLOGY, 30(1), 522–532. https://doi.org/10.1080/09540105.2019.1597022

BibTeX 
@article{liao2019-209984,
  author = {Liao, Yun and Cui, Xueyan and Chen, Ge and Wang, Yuanshang and Qin, Guoxin and Li, Mingjie and Zhang, Xiuyuan and Zhang, Yudan and Zhang, Chan and Du, Pengfei and Yan, Feiyan and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Wang, Jing and Zhang, Chenghui and Jin, Maojun},
  title = {Simple and sensitive detection of triazophos pesticide by using quantum dots nanobeads based on immunoassay},
  journal = {FOOD AND AGRICULTURAL IMMUNOLOGY},
  year = {2019},
  volume = {30},
  number = {1},
  pages = {522--532},
  issn = {0954-0105},
  doi = {10.1080/09540105.2019.1597022},
  url = {https://akademik.atauni.edu.tr/yayinlar/209984-simple-and-sensitive-detection-of-triazophos-pesticide-by-using-quantum-dots}
}