The Antibacterial Activity Mode of Action of Plantaricin YKX against Staphylococcus aureus
Özet
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.We aimed to evaluate the inhibitory effect and mechanism of plantaricin YKX on S. au-reus. The mode of action of plantaricin YKX against the cells of S. aureus indicated that plantaricin YKX was able to cause the leakage of cellular content and damage the structure of the cell mem-branes. Additionally, plantaricin YKX was also able to inhibit the formation of S. aureus biofilms. As the concentration of plantaricin YKX reached 3/4 MIC, the percentage of biofilm formation in-hibition was over 50%. Fluorescent dye labeling combined with fluorescence microscopy con-firmed the results. Finally, the effect of plantaricin YKX on the AI-2/LuxS QS system was investi-gated. Molecular docking predicted that the binding energy of AI-2 and plantaricin YKX was −4.7 kcal/mol and the binding energy of bacteriocin and luxS protein was −183.701 kcal/mol. The ex-pression of the luxS gene increased significantly after being cocultured with plantaricin YKX, suggesting that plantaricin YKX can affect the QS system of S. aureus.
Anahtar kelimeler
Atıf için künye
Pei, J., Huang, Y., Ren, T., Guo, Y., Dang, J., Tao, Y., Zhang, Y., & Hassıbelnaby, A. M. A. (2022). The Antibacterial Activity Mode of Action of Plantaricin YKX against Staphylococcus aureus. MOLECULES, 27(13). https://doi.org/10.3390/molecules27134280
BibTeX
@article{pei2022-209971,
author = {Pei, Jinjin and Huang, Yigang and Ren, Ting and Guo, Yaodong and Dang, Jun and Tao, Yanduo and Zhang, Yonggui and Hassıbelnaby, Abdelaty Mostafa Abdelaty},
title = {The Antibacterial Activity Mode of Action of Plantaricin YKX against Staphylococcus aureus},
journal = {MOLECULES},
year = {2022},
volume = {27},
number = {13},
issn = {1420-3049},
doi = {10.3390/molecules27134280},
url = {https://akademik.atauni.edu.tr/yayinlar/209971-the-antibacterial-activity-mode-of-action-of-plantaricin-ykx-against}
}