Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos

Yan, Mengmeng; Chen, Ge; She, Yongxin; Ma, Jun; Hong, Sihui; Shao, Yong; Hassıbelnaby, Abdelaty Mostafa Abdelaty; Wang, Miao; Wang, Shanshan; Wang, Jing

doi:10.1021/acs.jafc.9b03401

Makale Tam Makale Uluslararası 2019

Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos

Mengmeng Yan, Ge Chen, Yongxin She, Jun Ma, Sihui Hong, Yong Shao, Abdelaty Mostafa Abdelaty Hassıbelnaby, Miao Wang, Shanshan Wang, Jing Wang

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Cilt 67 · Sayı 34 · ss. 9658–9666 · 28.08.2019

DOI ile aç

Özet

Copyright © 2019 American Chemical Society.The biomimetic enzyme-linked immunosorbent assay (BELISA) is widely used for detection of small-molecule compounds as a result of low cost and reagent stability of molecularly imprinted polymers (MIPs). However, enzyme labels used in BELISA still suffer some drawbacks, such as high production cost and limited stability. To overcome the drawbacks, a biomimetic nanozyme-linked immunosorbent assay (BNLISA) based on MIPs and nanozyme labels was first proposed. For nanozyme labels, platinum nanoparticles (PtNPs) acted as peroxidase by catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into an ideal surface-enhanced Raman scattering (SERS) marker. Blue TMB2+ and bovine serum albumin (BSA)-hapten showed superior selectivity when competing with targets for binding sites on MIPs, named the Pt@BSA-hapten probe. The BNLISA method was employed to detect triazophos with a limit of detection of 1 ng mL-1 via colorimetric and SERS methods. Replacing traditional enzymes with nanozymes for combination with MIPs may bring about a new prospect for other compound analyses.

Anahtar kelimeler

horseradish peroxidase Triazophos Nanozyme molecular-imprinted polymer BNLISA

Atıf için künye

Yan, M., Chen, G., She, Y., Ma, J., Hong, S., Shao, Y., Hassıbelnaby, A. M. A., Wang, M., Wang, S., & Wang, J. (2019). Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 67(34), 9658–9666. https://doi.org/10.1021/acs.jafc.9b03401

BibTeX

@article{yan2019-209963,
  author = {Yan, Mengmeng and Chen, Ge and She, Yongxin and Ma, Jun and Hong, Sihui and Shao, Yong and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Wang, Miao and Wang, Shanshan and Wang, Jing},
  title = {Sensitive and Simple Competitive Biomimetic Nanozyme-Linked Immunosorbent Assay for Colorimetric and Surface-Enhanced Raman Scattering Sensing of Triazophos},
  journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  year = {2019},
  volume = {67},
  number = {34},
  pages = {9658--9666},
  issn = {0021-8561},
  doi = {10.1021/acs.jafc.9b03401},
  url = {https://akademik.atauni.edu.tr/yayinlar/209963-sensitive-and-simple-competitive-biomimetic-nanozyme-linked-immunosorbent-assay}
}