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Makale Tam Makale Uluslararası 2015

Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

Semin Park, Chul-Soo Park, Sung Joong Lee, Boseok Cha, Young Ah Cho, Yi Song, Eun Ae Yu, Gon-Sup Kim, Jong Sung Jin, Abdelaty Mostafa Abdelaty Hassıbelnaby, H.A. El-Banna, Ahmet Hacımüftüoğlu, Jae-Han Shim, Sung Chul Shin

Biomedical Chromatography Cilt 29 · Sayı 4 · ss. 612–618 · 01.04.2015
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Özet

© 2014 John Wiley & Sons, Ltd. In the present study, an effective high performance liquid chromatography-tandem mass spectrometric (HPLC/MS/MS) method was developed and validated to simultaneously determine bupropion (BUP), quetiapine (QUE) and escitalopram (ESC) in human plasma using carbidopa as the internal standard. Chromatographic separation was achieved on a Waters Sun Fire C 18 column using reversed-phase chromatography. The MS/MS experiment was performed in positive ion multiple reaction monitoring mode to produce product ions of m/z 240.3→184.2 for BUP, 384.2→253.1 for QUE, 325.3→109.3 for ESC and 227.2→181.2 for the internal standard. The method showed good linearity (R 2 ≥0.997), precision (relative standard deviation ≤7.5%), satisfactory intra- and interday accuracy (88.4-113.0%) and acceptable extraction recovery (87.2-115.0%), matrix effect (84.5.5-108.7%) and stability (92.3-103.5%). The method was successfully applied to determine the concentrations of BUP, QUE and ESC in human plasma samples.

Anahtar kelimeler

LC-MS/MS MS MAJOR DEPRESSIVE DISORDER LC quetiapine bupropion escitalopram

Atıf için künye

Park, S., Park, C., Lee, S. J., Cha, B., Cho, Y. A., Song, Y., Yu, E. A., Kim, G., Jin, J. S., Hassıbelnaby, A. M. A., El-Banna, H., Hacımüftüoğlu, A., Shim, J., & Shin, S. C. (2015). Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma. Biomedical Chromatography, 29(4), 612–618. https://doi.org/10.1002/bmc.3322

BibTeX 
@article{park2015-209960,
  author = {Park, Semin and Park, Chul-Soo and Lee, Sung Joong and Cha, Boseok and Cho, Young Ah and Song, Yi and Yu, Eun Ae and Kim, Gon-Sup and Jin, Jong Sung and Hassıbelnaby, Abdelaty Mostafa Abdelaty and El-Banna, H.A. and Hacımüftüoğlu, Ahmet and Shim, Jae-Han and Shin, Sung Chul},
  title = {Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma},
  journal = {Biomedical Chromatography},
  year = {2015},
  volume = {29},
  number = {4},
  pages = {612--618},
  issn = {0269-3879},
  doi = {10.1002/bmc.3322},
  url = {https://akademik.atauni.edu.tr/yayinlar/209960-development-and-validation-of-a-high-performance-liquid-chromatography-tandem}
}