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Makale Tam Makale Uluslararası 2020

Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

Ge Chen, Maojun Jin, Jun Ma, Mengmeng Yan, Xueyan Cui, Yuanshang Wang, Xiuyuan Zhang, Hui Li, Weijia Zheng, Yudan Zhang, Abdelaty Mostafa Abdelaty Hassıbelnaby, Ahmet Hacımüftüoğlu, Jing Wang

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Cilt 68 · Sayı 2 · ss. 660–668 · 15.01.2020
DOI ile aç

Özet

Copyright © 2019 American Chemical Society.A competitive sensitive bio-barcode immunoassay based on bimetallic nanozyme (Au@Pt: Gold@platinum) catalysis has been designed for the detection of the pesticide parathion. Gold nanoparticles (AuNPs) were modified with single-stranded thiol oligonucleotides (ssDNAs) and monoclonal antibodies (mAbs) to form AuNP probes; magnetic nanoparticles (MNPs) were coated with ovalbumin (OVA)-parathion haptens as MNP probes, and bimetallic nanozyme (Au@Pt) nanoparticles functionalized with the complementary thiolated ssDNA were used as Au@Pt probes. The Au@Pt probes reacted with the AuNP probes through complementary base pairing. Further, parathion competed with MNP probes to bind the mAbs on the AuNP probes. Finally, the complex system was separated by a magnetic field. The released Au@Pt probes catalyzed a chromogenic system consisting of teramethylbenzidine (TMB). The bimetallic nanozyme-based bio-barcode immunoassay was performed on rice, pear, apple, and cabbage samples to verify the feasibility of the method. The immunoassay exhibited a linear response from 0.01 to 40 μg·kg-1, and the limit of detection (LOD) was 2.13 × 10-3 μg·kg-1. The recoveries and relative standard deviations (RSDs) ranged from 73.12 to 116.29% and 5.59 to 10.87%, respectively. The method was found to correlate well with data obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In conclusion, this method exhibits potential as a sensitive alternative method for the detection of a variety of pesticides, ensuring the safety of fruits and vegetables in agriculture.

Anahtar kelimeler

PROTEIN SOLID-PHASE EXTRACTION gold nanoparticles AMPLIFICATION BIOSENSOR PLATINUM NANOPARTICLES RESIDUES ORGANOPHOSPHORUS PESTICIDES AuNPs Bio-barcode Immunoassay ULTRASENSITIVE DETECTION Au@Pt bimetallic nanozyme parathion AU-AT-PT

Atıf için künye

Chen, G., Jin, M., Ma, J., Yan, M., Cui, X., Wang, Y., Zhang, X., Li, H., Zheng, W., Zhang, Y., Hassıbelnaby, A. M. A., Hacımüftüoğlu, A., & Wang, J. (2020). Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 68(2), 660–668. https://doi.org/10.1021/acs.jafc.9b06125

BibTeX 
@article{chen2020-209945,
  author = {Chen, Ge and Jin, Maojun and Ma, Jun and Yan, Mengmeng and Cui, Xueyan and Wang, Yuanshang and Zhang, Xiuyuan and Li, Hui and Zheng, Weijia and Zhang, Yudan and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Hacımüftüoğlu, Ahmet and Wang, Jing},
  title = {Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis},
  journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  year = {2020},
  volume = {68},
  number = {2},
  pages = {660--668},
  issn = {0021-8561},
  doi = {10.1021/acs.jafc.9b06125},
  url = {https://akademik.atauni.edu.tr/yayinlar/209945-competitive-bio-barcode-immunoassay-for-highly-sensitive-detection-of-parathion}
}