Inert matrix and Na4EDTA improve the supercritical fluid extraction efficiency of fluoroquinolones for HPLC determination in pig tissues
Özet
A supercritical fluid extraction method combined with high-performance liquid chromatography-fluorescence detection was developed for the determination of enrofloxacin, danofloxacin, and ciprofloxacin in pig muscle, lung, and kidney samples. The optimal SFE conditions were 80 degrees C, 300 kg/cm(2), 30% methanol for 40 min as a dynamic extraction time, in addition to 0.2 g Na(4)EDTA and 7.0 g sea sand in the extraction vessel. The use of Na(4)EDTA and sea sand on SFE extraction resulted in improvement of the recoveries of ciprofloxacin, a polar and hydrophilic compound, as well as enrofloxacin and danofloxacin. Overall, the recoveries ranged from 86.7 to 113.1% using the Na(4)EDTA/sea sand-assisted SFE extraction method. The Na(4)EDTA/sea sand-assisted SFE-HPLC-FLD validated method was successfully carried out in pig tissues, and proved to be specific, sensitive, reliable, and accurate. The method was also applied satisfactorily for accurate quantitative residue analysis in incurred pig tissues. (c) 2008 Elsevier B.V. All rights reserved.
Atıf için künye
Choi, J., Mamun, M. I. R., Hassıbelnaby, A. M. A., Kim, K. T., Koh, H., Shin, H., Kim, J., Lee, K. B., & Shim, J. (2009). Inert matrix and Na4EDTA improve the supercritical fluid extraction efficiency of fluoroquinolones for HPLC determination in pig tissues. TALANTA, 78(2), 348–357. https://doi.org/10.1016/j.talanta.2008.11.021
BibTeX
@article{choi2009-209940,
author = {Choi, Jeong-Heui and Mamun, M. I. R. and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Kim, Kyung Tae and Koh, Hong-Bum and Shin, Ho-Chul and Kim, Jin-Suk and Lee, Kang Bong and Shim, Jae-Han},
title = {Inert matrix and Na4EDTA improve the supercritical fluid extraction efficiency of fluoroquinolones for HPLC determination in pig tissues},
journal = {TALANTA},
year = {2009},
volume = {78},
number = {2},
pages = {348--357},
issn = {0039-9140},
doi = {10.1016/j.talanta.2008.11.021},
url = {https://akademik.atauni.edu.tr/yayinlar/209940-inert-matrix-and-na4edta-improve-the-supercritical-fluid-extraction-efficiency}
}