Myokine musclin alleviates lipid accumulation in 3T3-L1 adipocytes through PKA/p38-mediated upregulation of lipolysis and suppression of lipogenesis

Choi, Sung Woo; Oh, Heeseung; Park, Seung Yeon; Cho, Wonjun; Hassıbelnaby, Abdelaty Mostafa Abdelaty; Hacımüftüoğlu, Ahmet; Jeong, Ji Hoon; Jung, Tae Woo

doi:10.1016/j.bbrc.2022.12.056

Makale Tam Makale Uluslararası 2023

Myokine musclin alleviates lipid accumulation in 3T3-L1 adipocytes through PKA/p38-mediated upregulation of lipolysis and suppression of lipogenesis

Sung Woo Choi, Heeseung Oh, Seung Yeon Park, Wonjun Cho, Abdelaty Mostafa Abdelaty Hassıbelnaby, Ahmet Hacımüftüoğlu, Ji Hoon Jeong, Tae Woo Jung

Biochemical and Biophysical Research Communications Cilt 642 · ss. 113–117 · 29.01.2023

DOI ile aç

Özet

© 2022 Elsevier Inc.Musclin (MUS), an exercise-responsive myokine, has been documented to attenuate inflammation and enhance physical endurance. However, the effects of MUS on differentiation and related molecular mechanisms in adipocytes have not yet been studied. In this study, we found that treatment with MUS attenuated lipid accumulation in fully differentiated 3T3-L1 cells. Furthermore, MUS treatment enhanced lipolysis assessed by glycerol release, and caused apoptosis, whereas it reduced the expression of lipogenic proteins, such as PPARγ and processed SREBP1. Treatment with MUS augmented phosphorylated PKA expression, whereas suppressed p38 phosphorylation in 3T3-L1 adipocytes. H89, a selective PKA inhibitor reduced the effects of MUS on lipogenic lipid accumulation as well as lipolysis except for apoptosis. These results suggest that MUS promotes lipolysis and suppresses lipogenesis through a PKA/p38-dependent pathway, thereby ameliorating lipid deposition in cultured adipocytes. The current study offers the potential of MUS as a therapeutic approach for treating obesity with few side effects.

Anahtar kelimeler

Obesity Adipocyte Lipogenesis Lipolysis musclin Myokine

Sürdürülebilir Kalkınma Amaçları

SKA 3: Sağlıklı Bireyler

Atıf için künye

Choi, S. W., Oh, H., Park, S. Y., Cho, W., Hassıbelnaby, A. M. A., Hacımüftüoğlu, A., Jeong, J. H., & Jung, T. W. (2023). Myokine musclin alleviates lipid accumulation in 3T3-L1 adipocytes through PKA/p38-mediated upregulation of lipolysis and suppression of lipogenesis. Biochemical and Biophysical Research Communications, 642, 113–117. https://doi.org/10.1016/j.bbrc.2022.12.056

BibTeX

@article{choi2023-209884,
  author = {Choi, Sung Woo and Oh, Heeseung and Park, Seung Yeon and Cho, Wonjun and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Hacımüftüoğlu, Ahmet and Jeong, Ji Hoon and Jung, Tae Woo},
  title = {Myokine musclin alleviates lipid accumulation in 3T3-L1 adipocytes through PKA/p38-mediated upregulation of lipolysis and suppression of lipogenesis},
  journal = {Biochemical and Biophysical Research Communications},
  year = {2023},
  volume = {642},
  pages = {113--117},
  issn = {0006-291X},
  doi = {10.1016/j.bbrc.2022.12.056},
  url = {https://akademik.atauni.edu.tr/yayinlar/209884-myokine-musclin-alleviates-lipid-accumulation-in-3t3-l1-adipocytes-through}
}