Enhancing the Sensitivity of the Bio-barcode Immunoassay for Triazophos Detection Based on Nanoparticles and Droplet Digital Polymerase Chain Reaction
Özet
Copyright © 2019 American Chemical Society.An ultrasensitive bio-barcode competitive immunoassay method based on droplet digital polymerase chain reaction (ddPCR) was developed for the determination of triazophos. Gold nanoparticles (AuNPs) were coated with monoclonal antibodies (mAbs) and complementary double-stranded DNA (dsDNA), which included bio-barcode DNA and thiol-capped DNA. Magnetic nanoparticle (MNP) probes were constructed by modifying the MNPs with ovalbumin-hapten conjugates (OVA-hapten). The target pesticide and OVA-hapten on the surface of the MNP probes competed with the AuNP probes simultaneously, and then the bio-barcode DNA was released for quantification by ddPCR. The concentration of released DNA was inversely proportional to the concentration of pesticide to be tested. Under the optimum conditions, the competitive immunoassay exhibited a wide linear range of 0.01-20 ng/mL and a low detection limit of 0.002 ng/mL. Spike recovery tests were carried out using apple, rice, cabbage, and cucumber samples to verify the feasibility of the method. The recovery and relative standard deviations (RSDs) of the technique ranged from 76.9 to 94.4% and from 10.8 to 19.9%, respectively. To further validate the results, a linear correlation analysis was performed between the proposed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Consequently, the bio-barcode immunoassay based on nanoparticles and ddPCR, an ultrasensitive method, showed great potential for the determination of target pesticides in real samples.
Anahtar kelimeler
Atıf için künye
Cui, X., Jin, M., Zhang, C., Du, P., Chen, G., Qin, G., Jiang, Z., Zhang, Y., Li, M., Liao, Y., Wang, Y., Cao, Z., Yang, F., Hassıbelnaby, A. M. A., & Wang, J. (2019). Enhancing the Sensitivity of the Bio-barcode Immunoassay for Triazophos Detection Based on Nanoparticles and Droplet Digital Polymerase Chain Reaction. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 67(46), 12936–12944. https://doi.org/10.1021/acs.jafc.9b05147
BibTeX
@article{cui2019-209863,
author = {Cui, Xueyan and Jin, Maojun and Zhang, Chan and Du, Pengfei and Chen, Ge and Qin, Guoxin and Jiang, Zejun and Zhang, Yudan and Li, Mingjie and Liao, Yun and Wang, Yuanshang and Cao, Zhen and Yang, Feiyan and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Wang, Jing},
title = {Enhancing the Sensitivity of the Bio-barcode Immunoassay for Triazophos Detection Based on Nanoparticles and Droplet Digital Polymerase Chain Reaction},
journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
year = {2019},
volume = {67},
number = {46},
pages = {12936--12944},
issn = {0021-8561},
doi = {10.1021/acs.jafc.9b05147},
url = {https://akademik.atauni.edu.tr/yayinlar/209863-enhancing-the-sensitivity-of-the-bio-barcode-immunoassay-for-triazophos}
}