A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification

Zhang, Chan; Du, Pengfei; Jiang, Zejun; Jin, Maojun; Chen, Ge; Cao, Xiaolin; Cui, Xueyan; Zhang, Yudan; Li, Ruixing; Hassıbelnaby, Abdelaty Mostafa Abdelaty; Wang, Jing

doi:10.1016/j.aca.2017.10.032

Makale Tam Makale Uluslararası 2018

A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification

Chan Zhang, Pengfei Du, Zejun Jiang, Maojun Jin, Ge Chen, Xiaolin Cao, Xueyan Cui, Yudan Zhang, Ruixing Li, Abdelaty Mostafa Abdelaty Hassıbelnaby, Jing Wang

Analytica Chimica Acta Cilt 999 · ss. 123–131 · 01.01.2018

DOI ile aç

Özet

A simple and highly sensitive immunoassay based on a competitive binding and bio-barcode amplification was designed for detection of small molecules, triazophos. The gold nanoparticles (AuNPs) were modified with monoclonal antibodies and 6-carboxyfiuorescein labeled single-stranded thiol-oligonucleotides (6-FAM-SH-ssDNAs); the fluorescence of 6-FAM was quenched by AuNPs. Ovalbumin-linked haptens were coated on the bottom of microplate to compete with the triazophos in the sample for binding to the antibodies on the AuNP probes. The fluorescence intensity was inversely proportional to analyte concentration. Parameters of AuNP probes preparation and immune reaction were optimized. At the optimal conditions, the salting process was shortened to 1 h and 166 +/- 9 ssDNAs were loaded onto a single AuNP. The competitive fluorescence bio-barcode immunoassay was performed on water, rice, cucumber, cabbage and apple samples. The linear range of the method was 0.01-20 mu g L-1 and the limit of detection (LOD) was 6 ng L-1. The recovery and relative standard deviations (RSDs) ranged from 85.0 to 110.3% and 9.4-17.4%, respectively. Good correlations were obtained between the results of the developed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In conclusion, it is suggested that the competitive fluorescent bio-barcode immunoassay had the potential to be used as a sensitive method for detection of a variety of small molecules in various samples. (C) 2017 Elsevier B.V. All rights reserved.

Anahtar kelimeler

STABILITY ASSAY LC-MS/MS Fluorescence PROTEIN gold nanoparticles DNA detection pesticides HYBRIDIZATION Bio-barcode Immunoassay Triazophos ULTRASENSITIVE DETECTION Rolling circle amplification GC-MS/MS

Atıf için künye

Zhang, C., Du, P., Jiang, Z., Jin, M., Chen, G., Cao, X., Cui, X., Zhang, Y., Li, R., Hassıbelnaby, A. M. A., & Wang, J. (2018). A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification. Analytica Chimica Acta, 999, 123–131. https://doi.org/10.1016/j.aca.2017.10.032

BibTeX

@article{zhang2018-209783,
  author = {Zhang, Chan and Du, Pengfei and Jiang, Zejun and Jin, Maojun and Chen, Ge and Cao, Xiaolin and Cui, Xueyan and Zhang, Yudan and Li, Ruixing and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Wang, Jing},
  title = {A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification},
  journal = {Analytica Chimica Acta},
  year = {2018},
  volume = {999},
  pages = {123--131},
  issn = {0003-2670},
  doi = {10.1016/j.aca.2017.10.032},
  url = {https://akademik.atauni.edu.tr/yayinlar/209783-a-simple-and-sensitive-competitive-bio-barcode-immunoassay-for-triazophos-based}
}