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A sensitive bio-barcode immunoassay based on bimetallic Au@Pt nanozyme for detection of organophosphate pesticides in various agro-products

Ge Chen, Guangyang Liu, Huiyan Jia, Xueyan Cui, Yuanshang Wang, Dongyang Li, Weijia Zheng, Yongxin She, Donghui Xu, Xiaodong Huang, Abdelaty Mostafa Abdelaty Hassıbelnaby, Jianchun Sun, Haijin Liu, Yuting Zou, Jing Wang, Maojun Jin, Bruce D. Hammock

FOOD CHEMISTRY Cilt 362 · 15.11.2021
DOI ile aç

Özet

© 2021 Elsevier LtdOrganophosphate pesticides (OPs) are often used as insecticides and acaricides in agriculture, thus improving yields. OP residues may pose a serious threat, due to inhibition of the enzyme acetylcholinesterase (AChE). Therefore, a competitive bio-barcode immunoassay was designed for simultaneous quantification of organophosphate pesticide residues using AuNP signal amplification technology and Au@Pt catalysis. The AuNP probes were labelled with antibodies and corresponding bio-barcodes (ssDNAs), MNP probes coated with ovalbumin pesticide haptens and Au@Pt probes functionalized with the complementary ssDNAs were then prepared. Subsequently, pesticides competed with MNP probes to bind the AuNP probes. The recoveries of the developed assay were ranged from 71.26 to 117.47% with RSDs from 2.52 to 14.52%. The LODs were 9.88, 3.91, and 1.47 ng·kg−1, for parathion, triazophos, and chlorpyrifos, respectively. The assay was closely correlated with the data obtained from LC-MS/MS. Therefore, the developed method has the potential to be used as an alternative approach for detection of multiple pesticides.

Anahtar kelimeler

organophosphate pesticides AuNPs Bio-barcode Nanozyme Au@Pt

Atıf için künye

Chen, G., Liu, G., Jia, H., Cui, X., Wang, Y., Li, D., Zheng, W., She, Y., Xu, D., Huang, X., Hassıbelnaby, A. M. A., Sun, J., Liu, H., Zou, Y., Wang, J., Jin, M., & Hammock, B. D. (2021). A sensitive bio-barcode immunoassay based on bimetallic Au@Pt nanozyme for detection of organophosphate pesticides in various agro-products. FOOD CHEMISTRY, 362. https://doi.org/10.1016/j.foodchem.2021.130118

BibTeX 
@article{chen2021-209780,
  author = {Chen, Ge and Liu, Guangyang and Jia, Huiyan and Cui, Xueyan and Wang, Yuanshang and Li, Dongyang and Zheng, Weijia and She, Yongxin and Xu, Donghui and Huang, Xiaodong and Hassıbelnaby, Abdelaty Mostafa Abdelaty and Sun, Jianchun and Liu, Haijin and Zou, Yuting and Wang, Jing and Jin, Maojun and Hammock, Bruce D.},
  title = {A sensitive bio-barcode immunoassay based on bimetallic Au@Pt nanozyme for detection of organophosphate pesticides in various agro-products},
  journal = {FOOD CHEMISTRY},
  year = {2021},
  volume = {362},
  issn = {0308-8146},
  doi = {10.1016/j.foodchem.2021.130118},
  url = {https://akademik.atauni.edu.tr/yayinlar/209780-a-sensitive-bio-barcode-immunoassay-based-on-bimetallic-au-at-pt-nanozyme-for}
}